I want to make a stable gel with sodium alginate and calcium chloride. Thanks if anyone can tell me the protocol for preparing this hydrogel?

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Which software is better to write systematic review paper?

We have seen some authors use some software to write review paper. As obvious, the study of all published papers concerning our studying field is not possible and these software can help us to...

27 February 2021 6,296 1 View

Reaction condition to convert NH2 to COOH?

I need to couple Dopamine by NH2 end to silica nanoparticles coated by PEG-NH2, but first i need to convert the PEG-NH2 to COOH by using succinic anhydrous ... can you suggest a reaction condition ?

13 February 2021 2,768 4 View

Surface modification of silica nanoparticles with PEG?

I'm trying to coat my silica NP with diamine PEG, so i used GOPTS to add epoxy group on the surface of the NP then i'll add the PEG. I can not found protocol for SiNP coating PEG, most of the...

13 January 2021 4,915 4 View

How much pre-PCR dilution of mixed-template DNA extracted from pollen (honey) is appropriate for Metabarcoding?

I'm working on pollen DNA metabarcoding to identify the floral composition of honey. How much pre-PCR dilution of mixed-template DNA extracted from pollen (honey) is appropriate for the...

28 December 2020 9,132 1 View

Silica nanoparticles synthesis using Stober method with size rang 30-50 nm?

I'm preparing silica NP controlling the size by using different concentration of NH4OH. 1- 16.75 ml ethanol. 2-0.5 ml TEOS. 3- NH4OH (0.3- 0.5- 0.75- 1 ml) mixed for 24 hr then evaporate the...

27 November 2020 1,504 13 View

Which neuronal cell line contain D1R?

I'm formulating a drug delivery system that could stimulate Dopamine receptor, For that i need to choose cell line that contain D1R and could be simulated using my delivery system... I have read...

20 November 2020 9,104 2 View

Problems in PCR using Folmer primers, can you help please ?

Hello, I'm using the classic Folmer LCO1490 and HCO2198 primers for a venerid bivalve ADN for two months but the results are still negative, I can't see the bands. 1. I tried to change the...

03 November 2020 9,190 3 View

How to culture prokaryote and eukaryote from the gut of marine organism?

Info regarding the type of media and culturing condition would be really appreciated. Thanks

21 July 2020 6,937 1 View

Is there a method to recognize all species from a mixed PCR product?

Hi everybody, I used universal primers to find the origin of plant or plants made material by insect and now I have a mixed PCR product of several plants. Is there a method to recognize all...

29 November 2018 5,314 3 View

Calculating sample size for a multivariate regression in GPOWER?

Hello I currently have two dependent variables (with 4 measurements each) and two independent variables My question is how to calculate the sample size using Gpower for multivariate multiple...

21 November 2018 5,719 2 View

What is the production process of CaFe ferroalloys?

I looking for ferro calcium production process

03 March 2021 1,755 4 View

How do I increase the yield of my PCR product?

Hello, We would like to increase the yield of our PCR product. We are running a series of PCR reactions that is targeting ~1.1kb sequence. We begin each reaction with ~400pg of template DNA...

02 March 2021 4,029 3 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

How can I represent SDS-PAGE results for machine learning analysis?

I am trying to classify and analyze the results of an SDS-PAGE based array for bacterial detection using machine learning, but I have trouble finding the best way to represent the results with...

27 February 2021 9,176 3 View

Why my agarose gel staining sometimes looks not homogenic but like gradient (see the figure)?

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25 February 2021 8,140 3 View

EMSA troubleshooting. Protein-DNA complex not migrating, cold probe lane with multiple bands. Can anyone help?

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24 February 2021 8,325 1 View

Problem with ligating 2.8 kb insert into 8.5 kb plasmid?

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24 February 2021 6,310 3 View

How to manage western blot for proteomics data validation?

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23 February 2021 6,671 4 View

Alginate and silk fibroin hydrogels toxic to cells?

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23 February 2021 8,249 2 View

Diltuions for a Western blot?

I need help using the equation C1V1=C2V2 figuring out how much antibody to add to use for a western blot. The stock was origianlly 3.45 ng/ul but diluted 1:10 so now its 0.345 ng/ul. I need to run...

22 February 2021 9,901 3 View

Abdelkader BOUAZIZ

Dear Hajar Mohamadzade, please refer to the following documents. My Regards

https://doi.org/10.1016/S1389-1723(00)87103-0

https://doi.org/10.1155/2021/5510779

André R. Fajardo

Dear Hajar, the following reference can be helpful: https://pubs.rsc.org/en/content/articlelanding/2012/ra/c2ra20785k

Regards, André.