04 January 2022 4 8K Report

stable gel with sodium alginate and calcium chloride

More Hajar Mohamadzade's questions See All
Which software is better to write systematic review paper?

We have seen some authors use some software to write review paper. As obvious, the study of all published papers concerning our studying field is not possible and these software can help us to...

27 February 2021 6,296 1 View

Reaction condition to convert NH2 to COOH?

I need to couple Dopamine by NH2 end to silica nanoparticles coated by PEG-NH2, but first i need to convert the PEG-NH2 to COOH by using succinic anhydrous ... can you suggest a reaction condition ?

13 February 2021 2,768 4 View

Surface modification of silica nanoparticles with PEG?

I'm trying to coat my silica NP with diamine PEG, so i used GOPTS to add epoxy group on the surface of the NP then i'll add the PEG. I can not found protocol for SiNP coating PEG, most of the...

13 January 2021 4,915 4 View

How much pre-PCR dilution of mixed-template DNA extracted from pollen (honey) is appropriate for Metabarcoding?

I'm working on pollen DNA metabarcoding to identify the floral composition of honey. How much pre-PCR dilution of mixed-template DNA extracted from pollen (honey) is appropriate for the...

28 December 2020 9,132 1 View

Silica nanoparticles synthesis using Stober method with size rang 30-50 nm?

I'm preparing silica NP controlling the size by using different concentration of NH4OH. 1- 16.75 ml ethanol. 2-0.5 ml TEOS. 3- NH4OH (0.3- 0.5- 0.75- 1 ml) mixed for 24 hr then evaporate the...

27 November 2020 1,504 13 View

Which neuronal cell line contain D1R?

I'm formulating a drug delivery system that could stimulate Dopamine receptor, For that i need to choose cell line that contain D1R and could be simulated using my delivery system... I have read...

20 November 2020 9,104 2 View

Problems in PCR using Folmer primers, can you help please ?

Hello, I'm using the classic Folmer LCO1490 and HCO2198 primers for a venerid bivalve ADN for two months but the results are still negative, I can't see the bands. 1. I tried to change the...

03 November 2020 9,190 3 View

How to culture prokaryote and eukaryote from the gut of marine organism?

Info regarding the type of media and culturing condition would be really appreciated. Thanks

21 July 2020 6,937 1 View

Is there a method to recognize all species from a mixed PCR product?

Hi everybody, I used universal primers to find the origin of plant or plants made material by insect and now I have a mixed PCR product of several plants. Is there a method to recognize all...

29 November 2018 5,314 3 View

Calculating sample size for a multivariate regression in GPOWER?

Hello I currently have two dependent variables (with 4 measurements each) and two independent variables My question is how to calculate the sample size using Gpower for multivariate multiple...

21 November 2018 5,719 2 View

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24 February 2021 8,325 1 View

Problem with ligating 2.8 kb insert into 8.5 kb plasmid?

I am trying to clone 2 copies of the same mammalian gene into the pSF-CMV-Ub-Puro Ascl (contains HindIII, KpnI and NheI cut sites) plasmid. This is what the final product is supposed to look like...

24 February 2021 6,310 3 View

How to manage western blot for proteomics data validation?

Hi Everyone, I am a bigginer in western blotting and I am trying to use it for validation of my proteomics data. I have: two regulated proteins with molecular mass of 40 and 73 kDa one control...

23 February 2021 6,671 4 View

Alginate and silk fibroin hydrogels toxic to cells?

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Diltuions for a Western blot?

I need help using the equation C1V1=C2V2 figuring out how much antibody to add to use for a western blot. The stock was origianlly 3.45 ng/ul but diluted 1:10 so now its 0.345 ng/ul. I need to run...

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