Since most of the kit-based extracellular vesicle isolation protocols rely on PEG precipitation (I do not know if it is also true for exospine kit or not), Is it safe to apply them in vivo animal models?
Hi Soura,
PEG is although often considered with a low toxicity, the ability of this oligomer or polymer to bind with the protein and other biological molecules is quite high. Thus, such a subsequence could result an unpredictable effects. What makes using these molecules along with the precipitated vesicles challenging is that using the appropriate control might address another obstacle toward analysing the in vivo data.
The other drawback of using kits containing PEG reagents for the in vivo (i.e. animal studies) experiments could state an unfavourable responses by raising unnecessary inflammatory reactions, and increase the antigenicity of the isolated vesicles.
If the facility is approachable, it is recommended to use the ultracentrifugation, perhaps without a cost to mention with high experimental reliability.
Hope this helps!
Good luck in your research..
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