Ibrahim Hasan Yaseen

2 Questions 30 Answers 0 Followers

Questions related from Ibrahim Hasan Yaseen

Mammalian gene - Stable knockdown: which system(vector) to be recommended?

Hello We are planning to knockdown a gene of a transmembrane protein in different human (cancer) cell line. The stable knockdown is might be the best for the current experiments and plans, and we...

12 December 2015 2,617 14 View

What is the most efficient concentration of G418 for stable transfection of CHO cell lines?

I am working on CHO cells transfection (plasmid: pcDNA3.1). I used different concentrations (0.25-1.5mg/ml), but that didn't kill the cells. Neomycin Sulphate(G418) from GIBCO. Lot: 121361. cat....

05 May 2014 10,005 31 View