I want to find out an unknown chemotactic factor that is secreted by the cells. Can any one suggest me a method of doing so?

13 June 2019 0 7K Report

the cells secrete some unknown chemotactic factor in media due to which they aggregate. I want to analyse and find out the unknown factor. Can any one suggest me a procedure to do that?

Badges
Science topic

Similar topics
Names

More Shilpa Sarkar's questions See All

How to get links for copyrights for papers?

how to get links for copyrights for papers?

06 August 2024 7,410 1 View

How to retain amplicon yield after Ampure bead cleanup, following a 2-stage PCR protocol?

Hello all, I have been trying to follow a 2-stage PCR protocol used to amplify barcodes of a large yeast library, as per Nyugen et al. (2022) -...

30 July 2024 841 2 View

How to perform band structure calculation of a bulk, nonmagnetic superconductor with spin-orbit coupling using vasp?

I'm new to VASP and I'm trying to calculate the band structure of a bulk, nonmagnetic superconductor with a tetragonal crystal structure. I'm including spin-orbit coupling (SOC) in my...

09 July 2024 5,641 3 View

Do social development programs integrate women into climate change adaptation initiatives, especially within the framework of poverty reduction?

Gender, along with socio-economic and environmental factors, is a key determinant of vulnerability to climate change.

24 June 2024 8,437 2 View

Why the number of atoms is different in xyz file and POSCAR file?

am trying to make POSCAR file and xyz file using vasp for NbReSi compound using VESTA. However, I notice that the number of atoms for xyz file is given as 41 whereas for POSCAR is it 36. Can...

17 April 2024 698 2 View

Alternative to nitrogenase?

I have seen nitrogen fixation in three heterotrophs, but cannot detect any nitrogenase in their genomes. Are there any alternative pathways that I can look into that may provide information about...

01 April 2024 5,641 0 View

XRD peak deviation?

In XRD analysis, when we move to a higher angle, like above 50 degrees, if I have two reference lines of different phases, one matches the peak and the other is towards the right of the peak with...

31 March 2024 1,812 2 View

What would be a standard protocol for termite sampling in sub-tropical Himalayan foothill forests?

My study area is in eastern Himalayan foothill landscape which is a tropical to sub-tropical forest; elevation ranges from 150 to 1300 meter. The forest is some parts dominated by sal, tick and...

21 March 2024 8,297 1 View

Help regarding Geometry Optimization of a bimetallic surface?

I am trying to perform a geometry optimization (GEO_OPT) of a FCC 111 Pt_{0.5}Au_{0.5} bimetallic surface (10x10x3) generated from ASE, with a lattice parameter of 3.9 A∘A∘ in CP2K. However, after...

18 March 2024 3,328 2 View

What is the effect of autoclaving PDA media twice consecutively? (without remelting, immediately after first autoclaving)?

What happens if the media is double autoclaved immediately after the first autoclaving? I am going to inoculate the Trichoderma; will it affect the sporulation of the Trichoderma?

25 February 2024 3,061 0 View

How to prepare bacterial conditioned media to study the effect of bacterial secretome?

I would like to investigate the effect of bacterial secretome on cancer cells. How do you prepare conditioned media from bacteria?? conditions- time points - media I am looking for the best way...

17 July 2024 3,785 9 View

Why is serum-free media used when making cancer cell conditioned media?

I am preparing cancer cell conditioned media to study the effects of substances secreted by cancer cells on other cells. In some papers, conditioned media is prepared using FBS-containing media...

11 July 2024 7,063 1 View

Comparison of tissue to serum MS data?

Hello, So I have mouse brain tissue MS data from one of my predecessors and I have human serum data from the hospital that I collaborate with. We are trying to find markers for the diagnosis of...

11 July 2024 8,484 0 View

ATG in pPICZalfphaA vector: Yes or Not in front of the gene of interest ... ?

Is it appropriate to place the ATG codon in front of the gene of interest, since there is a secretion signal that has its own ATG in front of this gene? I need my protein of interest to be...

10 July 2024 3,050 5 View

How it was possible for Dariusz Prokopowicz to get a Research Interest Score of 11,228 and 2,620,101 reads in ResearchGate?

What is the secret behind the success of the researcher named Dariusz Prokopowicz who has a "Research Interest Score" of 11,228 and has 2,620,101 reads in ResearchGate. How was it possible for him...

06 July 2024 2,963 0 View

Organoid with Claustro can be linked to LLM like chatGPT ?

Organoid with Claustro can be linked to LLM Neuroplatform - FinalSpark , finalspark.com/neuroplatform/ can be linked to chatGPT ? What to do with this ? Avatar Pilots ? Studying the case of...

28 May 2024 3,302 0 View

Does age of a bacterial culture have an impact on its DNA isolation?

Generally for bacterial DNA isolation we use a fresh culture of 2-5 days old. But if we use an older culture say 10-15 days old will it have any impact on the DNA content and the DNA isolation...

13 May 2024 3,061 11 View

Preparation of Pilocarpine for salivary secretion in Rats?

We are planning to collect saliva from rats for protein analysis. The issue is that the amount secreted is very low, which makes it difficult to analyze. To increase salivation, we purchased...

13 May 2024 4,631 0 View

Cryptosporidium gliding on non-cellular substrates?

Any pointers on how to get Cryptosporidium sporozoites to glide on non-cell substrates? We've tried oocysts from a couple of different sources (WaterBourne and the production lab at U of Arizona,...

07 May 2024 4,620 0 View

What are the medical uses of Atropine?

What are the medical uses of Atropine?The Role of Atropine in Modern Medicine: Indications, Admini... Atropine has several medical uses across different fields of medicine. Some of the primary...

04 May 2024 1,064 1 View