I want to estimate nitrate and oxalate content in leafy vegetables. What is the protocol? Should I use fresh leaves or dried leaves for extraction?

More Prithusayak Mondal's questions See All

What are the easy, simple and cost effective methods for making nano-formulations from solvent extract of any plant part?

01 February 2018 8,601 3 View

What do you think is the best suitable oil for dissolving curcuminoids, especially curcumin?

I want to make an o/w emulsion from plant extracts of turmeric. What is the best suitable oil for dissolving curcuminoids, especially curcumin? Can anybody send me a paper regarding solubility of...

01 February 2018 4,812 2 View

What is the relation of molecular weight of poly (ethylene glycol) with particle size in nano-encapsulation?

What is the relation of molecular weight of poly (ethylene glycol) with particle size in nano-encapsulation? Will PEG-1000 give smaller particle size as compared to PEG-400? What parameters should...

31 December 2017 1,146 3 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

Which transition metal salt precursor is best for doping? Why?

I would like to synthesize a transition metal doped TiO2 via solgel method. Hence, I would like to know which metal salt precursor to choose among Chloride, Nitrate, and Sulphate. A clear...

27 February 2021 4,285 3 View

Is there any one who can provide a donation of HPV detection and genotyping kits in exchange collaboration fors?

Warm greetings My name is Ayichew S. and I am doing my Ph.D. in Medical Microbiology (on Human papillomavirus). At this stage, I have almost completed a clinical sample Cervical swabs)...

25 February 2021 1,660 3 View

Is there any one who can provide a donation of HPV detection and genotyping kits in exchange collaboration forms?

Warm greetings! My name is Ayichew S. and I am doing my Ph.D. in Medical Microbiology focusing on Human papillomavirus). At this stage, I have almost completed a clinical sample (Cervical swabs)...

25 February 2021 3,612 3 View

What urea buffer can i use for biotagged protein extraction from streptavidin beads?

Hi all, I have been doing research on biotagged LDB1 proteins in Mel cells. I purify the proteins using M280 streptavidin beads. The yield is very low so I've been trying to optimize it by...

24 February 2021 5,749 3 View

What is the biochemistry of acidic solvents for phenolics extraction in strawberry?

I am using the Folin-Ciocalteu method for analysis of total phenolic content in ripe strawberries. I've read several protocol that suggest a methanol/H2O/formic acid (80:19:1) extraction solution....

22 February 2021 6,560 3 View

What reaction will occur when adding hexamine into ammonium nitrate in an acidic medium?

Hello, I am investigating properties of water in oil emulsion explosives. And adding hexamine (hexamethylenetetramine) into one of the phases that are used to make the emulsion should increase the...

21 February 2021 8,680 1 View

Protocol for membrane extraction E. coli?

Hello! I am interested in determining whether a poly-unsaturated fatty acid I have engineered E. coli to produce is being incorporated into the membrane of the cells or if it is simply a free...

21 February 2021 7,447 2 View

Using 1D CNN for pixel classification in an image?

I have a 3D Image of size 100x100x100. I have features (feature dimension 25) extracted for each pixel by considering a small pacth around it. So the input to 1D CNN is 100x100x100x25 reshaped to...

17 February 2021 941 4 View

Solid Phase Extraction of drug product containing nanoparticles ?

Hi Everyone, I am trying to develop an SPE method for the vaccine which contains Matrix-M (saponin Quillaja Saponaria ) which is in nano-particle form and size is around 40nm. These nanoparticles...

16 February 2021 1,389 2 View

Georgi Peev

Dear Colleague,

Researchers prefer to use leaves dried at defined conditions, because the fresh leaves loose their humidity very quickly and change their weight. Herbs and fruits are kept longer and sold after drying. However if your vegetables will be used fresh, you have to extract them fresh and to determine the water content in parallel sample by drying. As far as nitrates are concerned regarding the human health you must find the relevant state instruction and follow it strictly.

Jyotsna Dhanik

http://www.bioline.org.br/request?bk06018

http://www.academicjournals.org/journal/ISABB-JFAS/article-full-text-pdf/34A6FAE44870

Article Nitrate-N determination in leafy vegetables: Study of the ef...

Catherine M G C Renard

Dried for better stability; oxalate can be quantified by HPLC at 210 nm with a C18; nitrate as recommended by G. Peev use one of the standard methods, ISO or such..