If i will use calorimetric/sepc method bile pigments also give some color to reaction
i did whole exmoe sequencing using (NGS) ion Proton system .. i have the patients and control data... need suggestion and the pipelines filters for data analysis how to avoid and know the false...
07 August 2017 5,425 1 View
I usually use distilled water for primer dilution, i never got problem but one of GC rich (63%) primer getting degraded often. which one can be better for GC rich primer dilution TE or water if TE...
06 July 2017 3,043 26 View
hi i found a change in the morphology of min6 cells while doing GSIR. some cells even got lifted from the plate. is this common while performing the GSIR using Min6 cells?
08 September 2015 2,792 0 View
Hi I generally do GSIR by static method. Recently i found Perifusion method to study insulin release. So what is the difference between these two methods and which is more reliable? Thanks
06 July 2015 3,475 0 View
I have some frozen RNA samples in -80 and I want to do cDNA synthesis fallows qRT-PCR. I am using DNase I from genei i want use EDTA for deactivation of Dnase I after treatment with RNA thank you..
01 February 2015 9,911 4 View
When i check RNA purity the peak height at 260 or 280nm is less than the peak height at 230nm or other nm.
11 December 2012 1,976 10 View
Can anyone recommend a protocol for quantitative estimation of bile salts in bile sample?
02 March 2012 7,757 2 View
if i will run the gel of that PCR product did i will get any band ?
31 December 2011 9,216 15 View
Based on colorimetric method or any other method
31 December 2011 5,822 0 View
The problem is biological sample having bile pigments, it interfering while estimating the cholesterol..
31 December 2011 8,117 16 View
I was performing estimation of Total Soluble Sugar content in bacterial cultures by Anthrone method. Out of 9 samples, only one sample showed positive reading while others had a negative reading...
22 July 2024 9,576 4 View
In My article: The Extended Pedersen hypothesis (published, February 1988 Clinical Physiology and Biochemistry 6(2):68-73,) there is mistake of the name of first Author: it is not Catherine...
11 July 2024 1,801 2 View
I was wondering if it is possible to form a permanent open "ssDNA bubble" similar to a transcription bubble (>13 nucleotides) within E. coli. These criteria are important: 1. Open ssDNA...
07 July 2024 8,275 5 View
I have extracted essential oil from a medicinal plant and want to make several concentrations for some biological activities (Antidiabetic assay). How can i prepare the several concentrations to...
04 July 2024 8,100 4 View
Unluckily, just similar to an expensive car, your brain can be damaged if you ingest anything other than premium fuel.
23 June 2024 2,287 7 View
It is known that patients with desminopathy often die from pneumonia. Have pathomorphological studies of the lungs been performed in patients with desminopathy?
15 June 2024 1,355 7 View
Hi! I'm doing a renilla luciferase assay with coelenterazine and HEK293T cells transfected with AP-1 renilla luciferase. I'm wondering if the cells need to be lysed prior to adding coelenterazine...
13 June 2024 9,936 5 View
I have purchased Acetylcholiesterase from Electrophus electricus (electric eel), C3389-2KU, following details are written on the enzyme vial. Type-VI S lyophilized powder 200-1000 Units/mg...
31 May 2024 6,444 1 View
What is the difference between absorption and adsorption?
30 May 2024 4,447 27 View
I'm interested in knowing the differences in the effects caused by four different types of biochar: cantaloupe leaves/stems, onion leaves/stems, grape peels/stems/leaves and asparragus...
28 May 2024 4,907 0 View