Hi Parnian,

Standard polyacrylamide gels used for SDS-PAGE have limitations in resolving proteins above 500 kDa. I think you should consider using agarose gel instead of polyacrylamide gel because agarose gel has a larger pore size, allowing for better separation of high molecular weight proteins like yours. I am unaware of any commercially available protein ladders that reach the high molecular weight range of 700 kDa. Alternative techniques like Native PAGE or immunoprecipitation followed by western blotting might be better suited for studying a 700 kDa protein.

Dear Dr. Parnian Habibi

For the protein ladder, you may refer to the link below. It consists of 8 protein bands ranging in molecular weight from ~20–1200 kDa.

https://tools.thermofisher.com/content/sfs/manuals/NativeMarkUnstainedProteinStnd_man.pdf

For the Western Blot condition involving high molecular weight proteins, you may refer to the link provided below.

https://www.researchgate.net/post/How_to_increase_the_efficiency_of_high_molecular_weight_proteins_in_western_blot_technique

Best Wishes

Malcolm Nobre

Malcolm Nobre Thank you so much for sharing this useful information.

Augustine Udefa Thank you for your recommendations.