I want to do a comparative analysis of wild plant vs callus through LC-MS. How to prepare samples for that???

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Abdelhak Maghchiche

For LC-MS analysis, use about 2 grams of dried wild plant and callus samples, extracting with methanol at a ratio of 1:5 to 1:10. After soaking overnight, filter and concentrate the extracts for comparison.

Athira V. Anand

Robina Kausar Ethanol is also a good solvent option for the extraction of phytochemicals from the plant samples