I want to check cell Monolayer Integrity on trans-well. is the Lucifer Yellow Permeability Assay is right method to be use? if yes, can you let me know the Cat no please?
Hi Xiangju,
You may check the link below regarding Lucifer Yellow Permeability Assay.
In our vascular permeability experiments, we check monolayer integrity using 70kDa FITC-Dextran: size of dextran determines whether it will permeate through the monolayer, so the size can be adjusted accordingly; the FITC gives the fluorescence signal.
Although I have not used Lucifer Yellow before, I think it works with the same principle as that of the FITC-dextran but requires a different fluorescence excitation/emission to measure the fluorescence signal. I guess in this case, there's no right or wrong assay for your peameability assay, whatever suits for your application that works is okay.
Sincerely,
John
http://csmedia2.corning.com/LifeSciences/media/pdf/protocol_DL_GT_035_Lucifer_Yellow_Permeability_Assay_using_Falcon_HTS_96_Multiwell_Insert_Systems.pdf
I agree with John, alternatively you can just measure the resistance of the cell monolayer.
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