POPGENE V1.32 (Yeh et al. 1997). for polymorphism %,  Shannon’s information index (I, LogBase Ľ e), Nei’s genetic distance matrix, gene diversity (He), genetic differentiation (Gst) and gene flow (Nm).

DARwin5 software (Perrier and Jacquemoud-Collet 2006). For dendrogram, Principal coordinate analysis

 Gen Al Ex V6 (Peakall and Smouse 2012). For analysis of molecular variance (AMOVA), Mantel test, correlation between genetic distance and geographic distance, PCA.

For SSR, also you could use STRUCTURE, For genetic structure or differentiation 

They are no so complicated to use. 

POPGENE V1.32 (Yeh et al. 1997). for polymorphism %,  Shannon’s information index (I, LogBase Ľ e), Nei’s genetic distance matrix, gene diversity (He), genetic differentiation (Gst) and gene flow (Nm).

DARwin5 software (Perrier and Jacquemoud-Collet 2006). For dendrogram, Principal coordinate analysis

 Gen Al Ex V6 (Peakall and Smouse 2012). For analysis of molecular variance (AMOVA), Mantel test, correlation between genetic distance and geographic distance, PCA.

For SSR, also you could use STRUCTURE, For genetic structure or differentiation 

They are no so complicated to use. 

thank you so much but I am facing problems in data entry. can you give my your email?

UPGMA is an efficient software to analyse the DNA DATA 

DARwin,Structure and NTSYS are most used softwares which accept binary as well as allelic data.Just create your data file in excel and all three can easily import this excel datasheet.