I want extract protein from seed and i do not know which solution exactly use. and how i prepare solution . There is alot of method . If it is possible guide me with detail. thanks
Raghad Mouhamad
- Prepare the seeds by removing the hulls, shells, or other unwanted materials. You can use mechanical methods such as cracking, dehulling, or milling to do this. You may also need to dry the seeds or adjust their moisture content before extraction.
- Grind the seeds into a fine powder using a blender, mortar and pestle, or coffee grinder. This will increase the surface area of the seeds and make the extraction easier.
- Add water to the seed powder and mix well. The amount of water depends on the type and quantity of seeds, but a general ratio is 10 parts water to 1 part seed powder. You can use distilled water or tap water, but make sure the water is at room temperature or slightly warm.
- Add some additives to the water-seed mixture to enhance the extraction. You can use citric acid, cysteine hydrochloride, polyethylene glycol, or mercaptoethanol as additives. These additives can help break down the bonds between the proteins and the seed matrix, and also prevent the proteins from aggregating or denaturing. The amount and type of additives depend on the type and quality of protein you want to extract, but a general guideline is to use 0.1% to 1% of the additive by weight of the seed powder.
- Stir the mixture well and let it stand for 15 to 30 minutes. This will allow the water to dissolve the proteins from the seeds. You can also use a shaker or a blender to speed up the process.
- Filter the mixture using a cheesecloth, a coffee filter, or a fine sieve. The liquid part is the protein extract, and the solid part is the seed residue. You can discard the residue or use it for other purposes, such as animal feed or compost.
- Store the protein extract in a clean container and refrigerate it until you need it. You can also freeze it for longer storage. The protein extract may contain some impurities, such as carbohydrates, lipids, or minerals, so you may need to purify it further if you want to use it for specific applications. You can use methods such as precipitation, dialysis, chromatography, or electrophoresis to purify the protein extract.
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Narmela Asefi
Thank you so much
I dried seed by freeze drier . and grind them without remove shells.
for extraction i prepare buffer
3.872 gr sodium phosphate dibasic heptahydrate+ 1.457 gr sodium phosphate mono basic hydrate + 0.1168gr EDTA + water = 400 cc
and adjusted pH to 7.8 with NaOH
this was my buffer solution .
0.5 gr sample + 1.5 cc buffer solution and centrifuge at -4 C with x12000g
I am not sure my protein extract is correct or not ?
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