How to interpret the RDF plots?

I have studied the structural changes of a protein in 3 different concentrations of ionic liquids (50 mM, 500 mM, and 1M). On analysis of the RDF plots, I find that the magnitude of the RDF plot...

05 October 2020 4,972 2 View

Which is the best way to find the number of water molecules around the protein?

Dear all, I am trying to calculate the number of water molecules at a distance of 0.35A from the protein. I used two gmx select GROMACS codes which have given me different answers. 1. gmx_mpi...

17 March 2020 9,117 3 View

How to interpret the RDF plot?

Hi, I am studying the stabilization of proteins using different solvents using GROMACS. Attached is the RDF of the cation (black) and anion (red) around the protein. I used "gmx_mpi rdf com rdf...

18 June 2018 7,466 3 View

Can the activity of proteins be studied using molecular dynamic simulations?

I have been studying the conformational changes and stability of proteins in different solvents using MolecularDynamic Simulations. Wonder whether changes in activity of enzymes could also be...

06 April 2018 9,153 4 View

What is the better cell line to choose for studying the effect of a particular mutation in inducing tumorigenesis: normal or cancer cell line?

I need to study the effect of a particular mutation that is hypothesized to be tumorigenic. So which cell line should I choose to study its effect: a normal cell line or a cancer cell line?

15 January 2018 2,384 3 View

Please help with geometry optimisation in GAMESS- shift in double bond

25 May 2017 2,299 6 View

Hello Researchers, I am looking for a list of Proliferation Markers in cancer cells for my work. Kindly help me to list a few.?

Poliferation markers are those genes which can be looked for proliferation in cancer cells.

23 April 2017 8,311 9 View

What happens to the DNA from sloughed off intestine epithelial cells when extracting bacterial DNA from mouse fecal samples?

I am currently using the PowerSoil DNA extraction kit from MoBio for DNA extraction from bacteria present in mouse fecal matter. I wanted to know how the sloughed off intestine epithelial cells...

21 August 2016 5,137 1 View

Why does PBS treated with o-phthaldialdehyde exhibit absorbance at 332 nm when it is only a blank with no amine groups?

12 July 2016 8,655 3 View

mRNA to protein expression?

I have been conducting a series of experiments where I transiently transfect plasmid with my gene of interest into HT29 cells. When I performed qRT-PCR on these cell samples, I can clearly see an...

03 December 2013 6,975 8 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Is there any test to determine the cardiovascular risk of patients with obesity? from the perspective of obesity?

We found tests to determine cardiovascular risk taking into account lipid profile, blood pressure, age, history of diabetes, other laboratories. But when weight is involved, there is a paradox. I...

02 March 2021 6,036 1 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

What SPSS analysis should I run with one IV (with two levels) and two DVs?

Hi, I am trying to figure out which test would be most appropriate to run for the following question: Is there a difference in task blood pressure between smokers and non-smokers? There are two...

01 March 2021 8,235 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

Dapi fluorescence protocol for colon cell lines ?

28 February 2021 2,534 2 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

26 February 2021 10,041 3 View

Has anyone had luck using cell lines other than HEK 293 (such as COS1) to package lentivirus?

I am looking to package lentivirus in endothelial cells but I am opposed to using 293 cells due to personal ethics regarding the source. I have done some reading and saw that COS1, COS7, & CHO...

25 February 2021 3,030 1 View