I see two bands in EGF stimulated MDA-MB-468 and Du145 western blot probed with total EGFR protein and I don't know why?

More Nahid Golabi's questions See All

Why there were a high percentage of nitrogen in biogas composition?

Hello everyone, I analyzed the composition of biogas that was gathered from 25 days of anaerobic digestion. in the result, I had a high percentage of oxygen (22%) and a high percentage of nitrogen...

28 August 2023 8,655 5 View

How much results of HACK kits can be different from the results that be obtained by titrimetrically method?

Hi, I compared the results of COD analysis by kits (Hack) and the results from the titration method on same substrate. Unfortunately, the results of kits were almost double respect to the...

31 May 2023 1,392 0 View

Is there any way to dope Nitrogen in Yttrium Iron Garnets?

I am searching for a way to dope Nitrogen in Yttrium Iron Garnets or substitute Oxygen atoms. Can anyone suggest some articles regarding this.

22 May 2023 1,802 1 View

How to setup a Zn ion battery in lab for research purpose?

Zn ion battery is an emerging research field. I developed some cathode materials for Zn ion batteries. To test these materials I need a Zn ion battery setup. Can any one suggest an article...

02 May 2023 1,451 0 View

How to calculate Health risks assessments of surface water?

Can anyone help me to understand the calculation of Health risks assessments of surface water. I have 4 sampling sites and 10 heavy metals for each site. whether I should calculate HQ and HI for...

13 April 2023 1,691 3 View

How can we compare toxicity induced by a compound in 2 different tissues by chemometrics analysis??

Hello every one We've done an experiment on As toxicity in 2 different tissues. we've evaluated oxidative stress, biochemical and other parameters. Now, we want to know, in which tissue, As has...

31 July 2022 9,553 0 View

What is the importance of small scale culture in making the endophytic fungi extract ?

What is the difference between the large scale culture and small scale culture in endophytic fungi culture ?

25 June 2022 5,413 2 View

Why thermal conductivity of stainless steel (AISI 304) increases with increasing temperature?

For most solid materials, the thermal conductivity k decreases with increasing temperature but for AISI 304 stainless steel it increases.

03 June 2022 3,852 1 View

Any one used ELISA based assay for Antibody affinity?

Any one has used ELISA based assay to determine the affinity of an antibody? I have a few questions regarding the assay and the raw data, if any one may be able to assist, please.

19 February 2022 3,518 3 View

How to calculate ERD/ERS and Task related coherence in movement task?

I have a 64-channel preprocessed data with EEGLAB, which is 24 seconds of rest and 24 seconds of ankle dorsiflexion, and this has been done 5 times in a session. I want to extract the mu and beta...

13 August 2021 7,181 0 View

Does anyone know what might be causing the smeared bands on my western blot?

I got these smeared bands quite often lately. We typically run the gel at 140V with a 10-12% gel and do a wet transfer at 220 mA for 1.5 hr in cold room. We also noticed some dirty spots/dots (see...

10 August 2024 7,480 3 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

How much total RNA concentration to be extracted from sorted plasma cells from bone marrow of C57BL/6 mice for RT-PCR ?

i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...

05 August 2024 8,835 1 View

Weak DAPI staining after immunohistochemistry - how to improve?

After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...

05 August 2024 9,637 2 View

If we are using snowball sampling technique, how do we justify the true representativeness of the sample statistically? is there any statistical test?

Are there any statistical methods to justify your sampling technique using SPSS or AMOS?

05 August 2024 9,153 4 View

The Curse of Evolution and Complexity?

Brain and body mass together are positively correlated with lifespan (Hofman 1993). The duration of neural development is one of the best predictors of brain size, and conception is the best...

05 August 2024 6,247 3 View

What are possible strategies can be used to analyze data under sequential explanatory mixed method approach?

Better ways to analyze the qualitative and quantitative data in a sequential explanatory mixed method approaches

04 August 2024 2,703 6 View

How to use Density Functional Theory to calculate carrier mobilities of solid system?

Hello, everyone. I have tried to determine carrier motilities of some materials, by Density Functional Theory, using Quantum ESPRESSO. There are a few methods to do it, like a package called...

04 August 2024 8,894 1 View

Does long-term culture of murine Monocytes result in macrophage differentiation?

Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...

04 August 2024 7,282 2 View