No title

02 January 2021 7,417 10 View

What could be the cause of the Glutathione peak migration (LC Dionex, Kintetex EVO C18, water phase) ?

Hello, I need a help from those of you who work on Kinetex EVO C18 with water + 0.2% TFA mobile phase (isocratic). The conditions are constant (temperatures, flow, column, conditioning time)....

16 December 2020 4,582 3 View

How does one combine the basis of Quantum Physics that the information cannot be destroyed with the GR statement that black holes destroy the info?

How does one combine the basis of Quantum Physics that the information cannot be destroyed with the GR statement that black holes destroy the info?

14 December 2020 7,845 19 View

Macrostudies using sysGMM?

Dear all, Could you recommend macroeconomic studies (hopefully published in good journals) that use system GMM or Arellano-Bond estimators? Thanks a lot!

10 December 2020 5,130 4 View

What do you think about the article in Scientific American that nobody knows how planes lift off and fly?

https://www.scientificamerican.com/article/no-one-can-explain-why-planes-stay-in-the-air/

07 December 2020 4,404 19 View

No title

07 December 2020 9,722 12 View

Why my Nyquist plot does not intersect the x-axis?

I have been measuring the conductivity of the biopolymer membrane by using the 2-electrodes connection and sometimes the plot intersects the a-axis and other times doesn't. The membrane was...

03 December 2020 8,471 1 View

GPower for Three-Way ANOVA analysis?

I want to explore if the amount of time (usage) spent on the Social Media IV1, with two levels , one is light and the other is heavy use, Iv2, body image with negative and positive levels, Iv3...

17 November 2020 4,405 3 View

DNA extraction from bacterial culture?

Is it an effective method to isolate DNA from Gram-negative bacteria with only the use of a boil and freeze-thaw protocol, without any chemical purification? Also, is there a media to maintain the...

06 October 2020 6,890 8 View

Is there an equation connecting wave function and entropy of the quantum system?

Is there an equation connecting the wave function and the entropy of the quantum system?

27 July 2020 931 13 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Have someone successfully used the ER alpha (Santa Cruz sc-8002) antibody in immunostaining ?

Santa Cruz now recommends this new antibody (F-10) but I can't find a publication using this reference. I'm working on human et non human primate hypothalamus and so far, it doesn't work. Is...

02 March 2021 10,003 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Does anyone know an alternative product for cardiac Troponin T antibody for Immunofluorescence (Thermo # MA512960)?

We have been using Thermo Fisher anti-cardiac Troponin T antibody for immunofluorescence staining in FFPE mouse heart sections but it was recently discontinued. The literature shows it to be the...

01 March 2021 4,937 1 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

How vaccines of covid 19 work?

Millions ask how vaccines of covid 19 be successful and safe, its here to explain generally how vaccine of pfizer work ,mRNA vaccines teach our cells how to make a protein that will trigger an...

28 February 2021 2,723 4 View

How does an antibody have full intact antibody + half antibody?

Hello, I was just wondering how does a antibody have full intact antibody + half antibody? Is it because of the drug conjugation?

28 February 2021 1,577 1 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View

Does anyone use antinuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples?

I am looking for anti-nuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples. Any suggestions highly appreciated. Thank you.

28 February 2021 6,512 3 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View