29 September 2024 1 2K Report

I need your recommendations for this issue.

More Abdelsalam Zidan's questions See All
Is there STZ ideal dose for type 2 diabetic model in rats ?

Hi, I am working on developing type 2 diabetic rats (Sprague Dawley) using High-fat diet followed by a low dose of STZ injection I.P (30 mg/kg ). so those who have previous experience with this...

01 February 2023 1,840 3 View

Would you recommend hexane as a solvent?

I have a compound that does not dissolve when I try to dissolve it with DMSO to determine its cytotoxic effect on cell lines (DMSO is not as toxic as 1%, but 0.5% is recommended). I'm thinking to...

13 December 2022 6,621 1 View

Hi everyone, to growth and prolongs viability of cells in culture, how much non-essential amino acids solution (concentrate X100) must be in cell cul?

Hi everyone, to growth and prolongs viability of cells in culture, how much non-essential amino acids solution (concentrate X100) must be in cell culture medium?

23 February 2022 8,266 2 View

What is the best protocol for extraction of total oligosaccharides from wheat flour?

Good day, We are running a project where we are profiling oligosaccharides and monosaccharides from wheat flour samples. Our aim is to extract oligosaccharides directly from wheat flour for...

08 January 2022 4,658 4 View

I want to collaborate with a crop researcher from Germany?

Scientific cooperation

10 October 2021 3,819 0 View

Plasmid amplification in S2 cell?

When co-transfect S2 cell with selection vector (pCoHygro or pCoBlast ) to make a stable cell line, does the gene integrate into the genome or just like E.coli? How do the plasmids amplify within...

09 February 2021 5,777 1 View

While synthesizing hollow mesoporos silica nanoparticles, I do calcination to remove CTAB. The Silica powder turns into black, any idea why?

I follow the procedure mentioned here 10.1002/smll.201200962 At the end, I use muffle furnce at 550 C for 6 hours to remove CTAB. After calination, there was a significant difference in the...

16 October 2020 6,940 4 View

When you have a protein as a start, What are the best bioinformatics tools to identify candidate ligands?

There are many tools to identify the protein target for a ligand. When I start with a protein, what are the best tools to figure out the possible chemical ligand for this protein?

09 August 2020 9,177 3 View

Pschycological characteristics of junior guidence and sport selection?

I need recommendations for questionnaires that can be used to measure pschycological readiness for youth to participate in certain sport. therefore, it can be used to select youth and guide them...

13 July 2020 2,626 0 View

Why my negative control samples give positive signal (ELISA)?

I am using 2 HB Immulon plates, I coat some wells (coated) with bacterial antigen 10^9/ml (60C/1h inactivated bacterial antigen) and I left some wells with no antigen (uncoated). so coated vs...

01 May 2020 6,006 0 View

Similar questions and discussions
Which type of compound does lamda max of 218 indicate in a uv-vis spectrum of a partially purified compound through column and TLC?

A crude extract of fungal culture using EtOH was subjected to column and TLC and partially purified compound was obtained. UV vis spectrum of the compound/s has max absorbance at 218nm. The...

11 August 2024 9,801 2 View

Can you connect an HPLC to a Mass Spec only at a certain time point?

Can anyone explain this method? Especially the last statement where it says only at 1.5 to 2.5mins was the MS/MS connected to the UPLC. How is that possible, is it a feature in this specific...

11 August 2024 8,141 3 View

RNA Extraction Using Hot Borate Method No Longer Working?

I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...

08 August 2024 9,882 2 View

Can I use a HisTRAP column for affinity chromatography?

I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...

07 August 2024 505 3 View

Hi, please what is the best tool on Aspen plus that can help me get the best inlet temperature and pressure for cryogenic distillation?

I am trying to recover liquid CO2 from a mixture of 0.6 CO2, 0.3 N2, and 0.1 O2. My aim is to recover about 99% liquid CO2 at the bottom of the column and make sure the amount of gaseous CO2...

06 August 2024 4,611 0 View

How to clean the CAD detector?

The module of the CAD detector is corona Veo. It has been idle for a long time. And it was not well maintained before idle. I have replaced the gas filters and flushed the system with a 50/50...

01 August 2024 7,805 0 View

How to perform EEG source analysis on each trial of data separately?

Hello Everyone I have a question about structure for connectivity analysis on sources. My goal: preprocess and cut data into trials create headmodels, using template MRI file perform source...

30 July 2024 2,744 1 View

Screening of EPS producer LAB?

if you have MRS broth already prepared, what is the manner you will follow for preparing hyper-sweet medium for screening of EPS producer LAB? what the concentration of saccharose or glucose you...

26 July 2024 6,606 0 View

A positively charged and 10x his-tagged protein that doesn't bind to any chromatographic resin?

Hello everyone, I am currently working on a protein that is 10x his-tagged and positively charged (predicted pI=10.16). But when I tried to use Ni-NTA column to purify the protein, it's not...

24 July 2024 7,293 4 View

What is the Principle of Cysteine-carbazole-sulfuric acid reaction with glucose?

I have been looking for the principle of Cysteine-carbazole-sulfuric acid reaction in research papers and books, but couldn't find it. This reaction is mainly used for the quantification of...

23 July 2024 3,891 0 View

Our partners will collect data and use cookies for ad personalization and measurement. Learn how we and our ad partner Google, collect and use data. Agree & close