26 September 2024 1 9K Report

I am working on a project involving tissue-resident memory T cells in the thyroid, and CD69 is a key marker for these cells. However, I’ve struggled to find a CD69 antibody that works effectively for immunohistochemistry on paraffin-embedded (IHC-P) mouse thyroid tissue. I am particularly interested in antibodies that have been validated in similar studies. If anyone has experience with a specific CD69 antibody for this application, your insights would be extremely helpful.

More Solmaz Roshanmehr's questions See All
Why I get vector-insert concatemer during cloning?

Hi. I successfully cloned an insert into a plasmid vector and subsequently transformed it into E. coli. However, upon sequencing, I discovered that the insert-plasmid sequence is repeated two...

25 April 2024 5,493 4 View

Has the name of Influenza viruses been entered correctly into the NCBI?

I aligned my influenza virus sequence using NCBI BLAST. Some segments belong to H9 strains, while others are more similar to H5 and H7. Indeed, the results indicate similarity of PB1, for example,...

14 November 2023 5,493 2 View

Drawing phylogenetic tree?

Hello How can I design my phylogenetic tree like this sample? I mean the name of each sample is characterized with color rather than their name?

06 October 2023 7,744 4 View

Does DNA extraction need to be sterile?

Hello Does eukaryote DNA extraction need to perform in sterile condition with sterile (TE) buffer? And what about plasmid extraction? I read somewhere that plasmid DNA extraction does not need to...

16 July 2023 4,229 10 View

Why i see a band instead of smear after reverse crosslinking of Chromatin?

Hi, I am working with Arabidopsis and want to do ChIP-seq. I isolated chromatin from 14-day old seedlings and did sonification. After reverse crosslinking with 4 microliter of NaCL 5 M (ON), 2...

04 April 2023 6,789 0 View

How can I search for presence of a domain in a protein?

I am working on the oncolytic properties of some sequences in viruses. Now I am going to search for any possible Bcl-2 homology domain that may be responsible for apoptotic feature of the...

23 March 2023 8,098 2 View

How can I filter some structures from 3D proteins?

I am going to download the 3D structure of a protein from Uniprot data bank and describe a specific part of this protein. However, for makes it more clear, I need to remove some structures (like...

12 February 2023 1,251 2 View

Is matrix protein of morbiliviruses considered as a membrane-anchored protein?

According to the structure of morbiliviruses, the M protein is located on the inner surface of the virus envelope. However, It can binds to the RNP complex and the intracellular regions of the...

16 January 2023 9,605 2 View

Hello.Can anyone help me to identify mineral and environment in the following thin sections?

and if you see in book or article please say. thankyou

27 November 2022 2,046 1 View

Can you suggest a method for assay of catalase enzyme activity?

hello I will be thankful if anyone introduces a protocol for the assay of catalase enzyme activity.

30 October 2022 8,129 1 View

Similar questions and discussions
Is there a problem with my RNA pellet?

Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...

11 August 2024 7,082 3 View

Mouse CD3 antibody sequence?

Can anyone share any reference that gives mouse specific CD3 antibody sequence. I have been searching for this since few days and no luck. Thank you very much

08 August 2024 9,157 4 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

Can I use a HisTRAP column for affinity chromatography?

I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...

07 August 2024 505 3 View

Microtome tissue sectioning - How to prevent chatter of paraffin tissue specimen sections?

Hello everyone, I have recently started using the microtome device for sectioning of paraffin-embedded mice lung. While I had some success in sectioning and observing proper ribbons, some of my...

06 August 2024 666 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

Can I multiplex a mouse monoclonal and rat primary for IHC (mouse brain tissue)?

Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...

06 August 2024 9,710 1 View

How much total RNA concentration to be extracted from sorted plasma cells from bone marrow of C57BL/6 mice for RT-PCR ?

i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...

05 August 2024 8,835 1 View

Do you have good tips for seaweed tissue preservation in the field for post RNA extraction?

I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...

04 August 2024 501 2 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

Our partners will collect data and use cookies for ad personalization and measurement. Learn how we and our ad partner Google, collect and use data. Agree & close