Is the first clean up necessary in Illumina MiSeq Library Preparation?

I'm trying to streamline my metagenomics protocols for my lab. I have previously followed the 16S protocol...

16 September 2020 5,234 6 View

How do l do a floral extraction using polar and non-polar solvent ?

I have been struggling all week to find a decent paper with a protocol or extraction methods on how to extract floral compounds from bell pepper flowers using a (polar solvent) water and a...

20 February 2020 2,518 3 View

How do l do a floral extraction using polar and non-polar solvent ?

I have been struggling all week to find a decent paper with a protocol or extraction methods on how to extract floral compounds from bell pepper flowers using a (polar solvent) water and a...

01 February 2020 482 2 View

How do l send My Fusarium lactis DNA for sequencing?

I am hope to perform a PCR reaction on my targeted gene. Then do a gel electrophoresis and also purifying my DNA using Nucleospin Gel and PCR Clean- up kit. I am hoping to send my DNA for...

22 October 2019 653 3 View

I need to culture Fusarium lactis on PDA agar. Does anybody got a protocol for PDA agar to culture fungus?

I am a plant science student working on Fusarium lactis which affcets peppers in glasshouses?

09 October 2019 2,379 3 View

How do l send My Fusarium lactis DNA for sequencing?

I am hope to perform a PCR reaction on my targeted gene. Then do a gel electrophoresis and also purifying my DNA using Nucleospin Gel and PCR Clean- up kit. I am hoping to send my DNA for...

09 October 2019 2,679 4 View

Do many authors of scientific papers consider it imperative to obtain permission from a source, prior to citation as a "personal communication"?

I pose this question because it recently came up for a colleague who got some pushback after citing another colleague as a "personal communication", regarding field observations of a rare species...

10 September 2018 2,517 9 View

Non specific bands from qPCR - I've tried pretty much everything. Any ideas?

06 September 2016 4,765 16 View

How many trypan blue assays do I need to have meaningful data?

29 June 2016 1,488 3 View

Is there a required annealing temperature for the creation of silicon dislocation loops?

I have some silica samples that were implanted with aluminium and bismuth and annealed at 500C. They show emission that I'm fairly sure is silicon dislocation loop emission and I believe the...

01 February 2016 2,009 2 View

How climate change will influences human and animal behavioral change?

The role of climate change influencing the human behavior as well as economics interest

01 June 2021 0 0 View

flexibility of polymers chain

effect of double bond on the flexibility of polymers

07 April 2021 0 0 View

Formulation of polymer color flakes

I am writing to request some information for making polymer color flakes. I would appreciate the help.

17 March 2021 0 0 View

Which is the best place in the world for the plant biotechnology research?

I am searching for a good place for the Post Doc,

02 March 2021 4,053 3 View

Please suggest a good text book for "Research Methodology in Life Sciences". Which book would be good for PhD Scholars?

Please provide Book Title and author name

02 March 2021 9,059 3 View

How to grow E.coli on the low salt LB agar plate with 50 or 100ug/ml of Blasticidin?

Hello, I would like to ask how to grow E.coli which resists 50 or 100ug/ml of Blasticidin. I have tried many methods by using low salt Luria-Bertain(LB) agar plate. However, no colonies grow. Let...

01 March 2021 2,027 2 View

Which bacteria are suitable for quorum sensing screening?

I want to find a quorum sensing inhibitor from plant extracts. Klebsiella pneumoniae, Moraxella catarrhalis, Staphylococcus aureus, and Streptococcus pneumoniae. I looked for these strains...

01 March 2021 7,675 3 View

Isolates of Hymenoscyphus fraxineus?

Dear Dr. Cai, my name is Simone Prospero and I work in the team of Phytopathology at the Swiss Federal Institute for Forest Snow and Landscape Research (WSL;...

01 March 2021 1,133 1 View

How to remove the residual PDMS on the silicon mold?

Incompletely cross-linked pdms are difficult to remove.

01 March 2021 798 3 View

Where to find data on trees in tropical agroforestry?

Hi, I'm looking for data (mainly related to management: growth rate, canopy size, soil and climate preferences, etc.) about tropical trees used in tropical agroforestry. Have you ever heard about...

28 February 2021 7,356 8 View