I sent 27 microbes for PCR and sequencing at a lab in Kaduna, Nigeria, but only 9 came back with sequences. Now I need them analysed to identify these 9. I wish I could send samples somewhere else with guarantee of good result feedback. Any ideas?
There are many free software programs available that you can use to construct phylogenetic trees using your sequence data. The two best (I have found) are MEGA (a free downloadable program- an explanation of how to use this program is here: http://www.formatex.info/microbiology4/vol1/736-743.pdf) or the TRex online server (http://www.trex.uqam.ca/).
As for sequencing I have always found Eurofins to be reliable (http://www.eurofins.com/)
Thanks a lot Mr Ryan. I have been trying blast at the NCBI website. It seems there some nittygritty details about using these engines. Experimented with the standard nucleotide blast, and seems d results varied from where I specify for microbial blasts - eeither for representative or complete genomes. Tried specifying the microbe genus too to see if there'd be a different outcome. D outcome was different. D question now is do I go with d standard blast without specifying any particular parameter (excepting the megablast option. For highly similar sequences) or not?
But will surely check out the links you'v sent and recommended. Still a rookie trying out all possibilities. 'Smiles'. God bless
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