I need some protocol to increase RNA integrity in my samples from Oreganum vulgare?

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Eric Daniel Gutiérrez-Pérez

I suggest TRIzol method. After the addition of 2-propanol, in RNA precipitation, you can incubate 1h at -80°C the sample to obtain the major concentration of RNA.

Ritesh Kumar

Hi Sumira

You can follow the CTAB for good quality RNA, alternatively you can use the Sigma plant RNA isolation kit (Spectrum).

http://www.nature.com/protocolexchange/protocols/208

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4435465/

https://www.ncbi.nlm.nih.gov/pubmed/18618437

http://staff.cimap.res.in/PublicationFiles%5CIndian_Journal_of_Experimental_Biology_43197.pdf

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3626780/

http://homepage.ntu.edu.tw/~jmhu/hulab/protocols/RNA%20isolationShu.pdf

Spectrum™ Plant Total RNA Kit

http://www.sigmaaldrich.com/catalog/product/sigma/strn10?lang=en®ion=IN

Sumira Jan

I have already tried Trizol - DEPC treatment but integrity of RNA still remains low.. Now I am working towards combination of methods in which LiCl has been used.