I need some protocol to increase RNA integrity in my samples from Oreganum vulgare?

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Different primers for same gene but different results in expression?

Hey everyone, I am currently doing my doctoral thesis at the department of pathology. I designed two different primers for my gene of interest having two different amplification sizes (130bp and...

17 February 2021 6,684 3 View

By which mechanism does potassium hyrdoxide works as an activator in the preparation of activated carbon?

Activated carbon is prepared from biomass using KOH as the activating agent. What is the possible reaction between KOH and the biomass?

14 February 2021 8,095 4 View

How to address SRMR (0.2 to 0.8) to make model fit?

Dear researchers, I want to update my knowledge regarding data analysis. I will be honored if you can help me to reduce in correcting SRMR in SEM_PLS. All the measures (AVE, CR, VIF, CA, HTMT) are...

09 February 2021 1,702 1 View

How to approach quantification of cytoplasmic immunofluorescent signal in adipocytes?

Any ideas on how to quantify a non-nuclear immunofluorescent signal in adipocytes? I had the idea to do a watershed on inverted image to "locate" the center of the empty space where lipid droplets...

05 January 2021 5,420 1 View

Are ther ways to quantify / measure the degree of loneliness?

Are there ways to quantify / measure / monitor the degree of loneliness of team members in a Covid-19 lockdown teleworking situation? Team dynamics over a longer period of teleworking and...

16 November 2020 4,212 9 View

Does anybody have experiences with FFF separation of PHB particles?

I am trying to separate suspension of PHB particles, stabilized with PVA. The DLS results show two populations with peak maxima about 150 nm and 380 nm. The Zeta potential was -3 mV (pH 7) across...

21 October 2020 6,551 1 View

What is the impact of the covid-19 pandemic on global warming?

Because of extensive lock up during the pandemic in many countries of the world, notably Europe, USA, Brazil, China and India, there was reduced use of hydrocarbons, especially due to much reduced...

15 October 2020 8,539 23 View

Is there any way to find RUL of bearing with incomplete dataset?

I am interested in finding RUL of bearing, which is currently in operation. I could find papers which uses run to failure datasets, which is not of my interest. Can anyone suggest methods or...

11 October 2020 7,962 7 View

Does anyone know of ontologies of the knowledge covered in US middle school and high school curricula?

For one of our projects we are looking for ontologies of middle and high school topics, such as math, science, history, and others. Is anyone aware of existing ontologies that are available for use?

10 October 2020 2,040 3 View

Multiple-Group Invariance with Categorical Outcomes AND correlated residuals?

Dear all, I have a question relating to the following paper: Dubravka Svetina, Leslie Rutkowski & David Rutkowski (2020) Multiple-Group Invariance with Categorical Outcomes Using Updated...

08 October 2020 3,142 3 View

What is the most Operating System (OS) which can be INSTALL on Tiny hard devices like Raspberry Pi (RPi) hardware ?

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03 March 2021 1,931 5 View

How to increase a model accuracy ?

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Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View

How can I integrate a routing protocol in a tiny device (e.g. Raspberry Pi)?

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01 March 2021 9,332 6 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

Why is the efficiency of viral mRNA electroporation in PBMC low?

Hello, I have been struggling with the electroporation of PBMC with HIV-I mRNA. Although the quality of the RNA generated is good, and the handling of the cells in meticulous, the frequency of...

27 February 2021 9,233 3 View

Is there any one who can provide a donation of HPV detection and genotyping kits in exchange collaboration fors?

Warm greetings My name is Ayichew S. and I am doing my Ph.D. in Medical Microbiology (on Human papillomavirus). At this stage, I have almost completed a clinical sample Cervical swabs)...

25 February 2021 1,660 3 View

Is there any one who can provide a donation of HPV detection and genotyping kits in exchange collaboration forms?

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25 February 2021 3,612 3 View

What urea buffer can i use for biotagged protein extraction from streptavidin beads?

Hi all, I have been doing research on biotagged LDB1 proteins in Mel cells. I purify the proteins using M280 streptavidin beads. The yield is very low so I've been trying to optimize it by...

24 February 2021 5,749 3 View

Reza Hadavi

you can test "RNeasy Plant Mini Kit" from Qiagen .

good luck

Eric Daniel Gutiérrez-Pérez

I suggest TRIzol method. After the addition of 2-propanol, in RNA precipitation, you can incubate 1h at -80°C the sample to obtain the major concentration of RNA.

Ritesh Kumar

Hi Sumira

You can follow the CTAB for good quality RNA, alternatively you can use the Sigma plant RNA isolation kit (Spectrum).

http://www.nature.com/protocolexchange/protocols/208

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4435465/

https://www.ncbi.nlm.nih.gov/pubmed/18618437

http://staff.cimap.res.in/PublicationFiles%5CIndian_Journal_of_Experimental_Biology_43197.pdf

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3626780/

http://homepage.ntu.edu.tw/~jmhu/hulab/protocols/RNA%20isolationShu.pdf

Spectrum™ Plant Total RNA Kit

http://www.sigmaaldrich.com/catalog/product/sigma/strn10?lang=en®ion=IN

Henah Mehraj Balkhi

Trizol method ... DEPC treatment should be strictly followed

Sumira Jan

I have already tried Trizol - DEPC treatment but integrity of RNA still remains low.. Now I am working towards combination of methods in which LiCl has been used.