Have a look on following URL

http://www.imtech.res.in/raghava/

http://www.imtech.res.in/raghava/gpsr/

some of the available mapping software are in these websites

http://www.epitope-informatics.com/

http://pepitope.tau.ac.il/overview.html

also read these articles to find different databases and software used by various researchers

1. Helper T Cell Epitope-Mapping Reveals MHC-Peptide Binding Affinities That Correlate with T Helper Cell Responses to Pneumococcal Surface Protein A

2. MIMOX: a web tool for phage display based epitope mapping

3. T-cell Epitope Mapping Strategies

4. Epitope Mapping Protocols (book)

THANKS A LOT for your information (M.Yasar)

Try Bepipred for B cell epitopes http://www.cbs.dtu.dk/services/BepiPred/

the excellent Immune epitope database resource http://tools.immuneepitope.org/main/index.html

and RosettaAntibody http://antibody.graylab.jhu.edu/

Good luck

Hope these would be useful- SEPPA, epimatrix, tepitope

Thanks so much guys...-it's so useful for my research

I would also go here:

http://immunax.dfci.harvard.edu/Tools/

You can also go for epitopia tool

If you want make mapping IgE epitope, SPADE is a good predictor tool. This tool was developed by Valenta

You might find the suite of tools available at the CBS homepage (http://www.cbs.dtu.dk/services/) useful. Look under "Immunological features". There is a tool for prediction/mapping of B-cell epitopes (DiscoTope) onto structure as well as a host of methods for MHC-I and MHC-II epitope prediction. Especially NetMHCpan, NetMHCIIpan and NetMHCcons might be of interest to you.

Have a look on following URL

http://www.imtech.res.in/raghava/

http://www.imtech.res.in/raghava/gpsr/

You can visit the following web site for Epitope prediction.

www.rajasekaran.net.in/tools/carbana.html

Carbana tool is used for prediction. Reference: Epitope Prediction Based on Carbon Content, S. Princeinal Suganthi, S. Shiney Valentina, S. Arul Mugilan & E. Rajsekaran, Int. J. Bioinfo., Pages: 25-27 (Vol.4 No.2 2011)

Thanks....all.

you also try with the Kohleskar and tongaokar method

http://www.vivo.colostate.edu/molkit/hydropathy/index.html

Although above link is old but worked nicely to determine hydrophobic and antigenic regions in a protein. Good luck.

My name is Fabian Leotteau, I have working with software madapper for videomapping, is excellent. You can see the software and how working with it. You can buy  it in   http://madmapper.com/  

In the web site you will found the you need about it   

Bepipred is good for B cell epitopes

http://www.cbs.dtu.dk/services/BepiPred/

Discotope and Ellipro

Hello, I recommend using the above algorithms and servers to generate a consensus antibody epitope prediction, then cross-reference and evaluate consensus predictions in light of relevant molecular information available for your target molecule(s). Further, 3-D structure will enhance epitope prediction and selection for further work, and structure modelling should be investigated if solved 3-D structures are not available. Good luck!

How to analyse the data output from Discotope

?

Warning! Unable to find the pdb entry 'ATOM 18 CG LEU A 23 10.257 55.244 72.035 1.00 0.69 C' Warning! Unable to find the pdb entry 'ATOM 19 CD1 LEU A 23 10.581 56.347 71.021 1.00 0.69 C' Warning! Unable to find the pdb entry 'ATOM 20 CD2 LEU A 23 9.616 55.866 73.284 1.00 0.69 C' Warning! Unable to find the pdb entry 'ATOM 21 N SER A 24 6.805 52.560 71.936 1.00 0.75 N' Warning! Unable to find the pdb entry 'ATOM 22 CA SER A 24 5.581 51.774 71.845 1.00 0.75 C' Warning! Unable to find the pdb entry 'ATOM 23 C SER A 24 4.834 52.062 70.570 1.00 0.75 C' Warning! Unable to find the pdb entry 'ATOM 24 O SER A 24 4.592 53.214 70.227 1.00 0.75 O' Warning! Unable to find the pdb entry 'ATOM 25 CB SER A 24 4.615 52.119 73.009 1.00 0.75 C' Warning! Unable to find the pdb entry 'ATOM 26 OG SER A 24 3.512 51.218 73.127 1.00 0.75 O' Warning! Unable to find the pdb entry 'ATOM 27 N ILE A 25 4.450 51.008 69.835 1.00 0.75 N' Warning! Unable to find the pdb entry 'ATOM 28 CA ILE A 25 3.791 51.116 68.548 1.00 0.75 C' Warning! Unable to find the pdb entry 'ATOM 29 C ILE A 25 2.627 50.143 68.545 1.00 0.75 C' Warning! Unable to find the pdb entry 'ATOM 30 O ILE A 25 2.462 49.334 69.459 1.00 0.75 O' Warning! Unable to find the pdb entry 'ATOM 31 CB ILE A 25 4.728 50.879 67.350 1.00 0.75 C' Warning! Unable to find the pdb entry 'ATOM 32 CG1 ILE A 25 5.067 49.396 67.078 1.00 0.75 C' Warning! Unable to find the pdb entry 'ATOM 33 CG2 ILE A 25 6.036 51.669 67.579 1.00 0.75 C' Warning! Unable to find the pdb entry 'ATOM 34 CD1 ILE A 25 5.747 49.204 65.723 1.00 0.75 C' Warning! Unable to find the pdb entry 'ATOM 35 N ALA A 26 1.777 50.177 67.503 1.00 0.83 N' Warning! Unable to find the pdb entry 'ATOM 36 CA ALA A 26 0.871 49.085 67.229 1.00 0.83 C' Warning! Unable to find the pdb entry 'ATOM 37 C ALA A 26 1.250 48.483 65.887 1.00 0.83 C' Warning! Unable to find the pdb entry 'ATOM 38 O ALA A 26 1.786 49.157 65.007 1.00 0.83 O' Warning! Unable to find the pdb entry 'ATOM 40 N PHE A 27 1.032 47.169 65.718 1.00 0.81 N' Warning! Unable to find the pdb entry 'ATOM 41 CA PHE A 27 1.466 46.450 64.538 1.00 0.81 C' Warning! Unable to find the pdb entry 'ATOM 42 C PHE A 27 0.472 45.311 64.289 1.00 0.81 C' Warning! Unable to find the pdb entry 'ATOM 44 CB PHE A 27 2.903 45.927 64.798 1.00 0.81 C' Warning! Unable to find the pdb entry 'ATOM 45 CG PHE A 27 3.602 45.636 63.515 1.00 0.81 C' Warning! Unable to find the pdb entry 'ATOM 46 CD1 PHE A 27 3.623 44.327 63.026 1.00 0.81 C' Warning! Unable to find the pdb entry 'ATOM 47 CD2 PHE A 27 4.200 46.666 62.769 1.00 0.81 C' Warning! Unable to find the pdb entry 'ATOM 48 CE1 PHE A 27 4.200 44.051 61.787 1.00 0.81 C' Warning! Unable to find the pdb entry 'ATOM 49 CE2 PHE A 27 4.770 46.390 61.519 1.00 0.81 C' Warnin