How can I isolate and characterise ACE from rabbit
At first please see the attachment. This experiment was performed for a period of 7 days (n=100). After 7 days, we found different level of survival rate (%) for each treatment. Now, I want to...
15 February 2021 9,145 2 View
The organo montmorillonite nanoclays modified with octadecylamine were used for the experiment. After acid treatment, the surface area will be increase. However, in my experiment, the surface area...
10 February 2021 1,581 3 View
Dear all, I am trying to optimize a bimetallic complex using # opt=tight b3lyp/lanl2dz scf=qc geom=connectivity. The program is running for around 5 days but the size of the .chk remains same...
05 February 2021 8,721 3 View
The recommended amount of DNA for restriction digestion is 1ug in a 50ul rx I've got around 500-600ng of DNA in a total volume of 35ul. Would I get satisfactory results with this amount of DNA for...
31 January 2021 729 6 View
Please share your experience on the effectiveness or challenges on online teaching during Covid-19 lock down. Share your experience about challenges, technology, online learning platform,...
26 January 2021 4,532 37 View
Recently I have generated LC-MS metabolomics data. After annotation, some have their compound name but either no KEGG id or KEGG id but no pathway. How to interpret those compounds? Additionally,...
18 January 2021 1,938 3 View
The current global pandemic, the climate crisis, the destruction of the oceans (with plastics, etc), and massive numbers of refugees are part of a set of serious global problems. It is clear...
13 January 2021 2,022 3 View
I want to remove a duplicate citation
01 January 2021 3,983 3 View
Hello, I am a newbie in geant4. I'm learning it playing with example. Sometimes, when I go to simulate an example code, it doesn't create an executable file (the green one in my ubuntu terminal)....
26 December 2020 9,482 3 View
Hello all, I want to do glycosaminoglycan (GAG) simulation in Gromacs. Initial structure and CHARMM topology for short chains can be obtained from Charmm GUI glycan modeler. But I have to take...
23 December 2020 6,092 2 View
Favourable conditions of base pressure, rate of deposition. Also, by which characterisation technique to be used to verify the thickness of the deposited film.
28 February 2021 3,640 3 View
I am trying to find the fraction metabolized of a drug by CYP and UGT enzymes. I am unable to get the values of fraction metabolized (Fm) directly from the literature. Can we calculate the Fm of a...
21 February 2021 5,379 2 View
21 February 2021 3,417 5 View
I am trying to set up a cortisol EIA using mouse anti-rabbit IGg as coating and a rabbit cortisol antibody as secondary ab. I am searching for a sensitive and specific cortisol Ab made in rabbit
20 February 2021 3,366 5 View
Hi, I'm asking from the point of view of designing a research plan: How long (approximately) can it take to determine the transition state structure of an enzyme substrate using kinetic isotope...
14 February 2021 2,610 1 View
Hello, I am trying to find kinetic information (such as rate of enzyme production etc.) on the expression of taq polymerase in E-Coli after inserting a recombinant plasmid. Any help would be...
12 February 2021 1,386 3 View
Hello! I want to determine the activity of my in house Reverse Transcriptase enzyme. For this, I have used Thermo's Enzcheck Reverse Transcriptase assay kit. I used commercial Thermo RT enzyme to...
08 February 2021 7,285 1 View
Hello, I have recently run a western of Vero cells for ACE2. I detected ACE2 at the correct size, but it presents as a double band. The sample was prepared by lysing cells in 1X Laemmli...
07 February 2021 6,973 3 View
07 February 2021 2,006 1 View
Hi everyone, I need to clone in pGEM-T vector the result of amplifying a metagenomic library by PCR with a high fidelity polymerase but I only want to clone the fragments of an specific size...
04 February 2021 6,051 8 View
