I am working with a protein and trying to resolve it on Blue-Native PAGE. Can somebody suggest be a recipe for it?

I have been trying to perform Blue-Native PAGE using procedure from doi:10.1038/nprot.2006.62. However, the stacking gel does not solidifies even after two hours, at room temperature. Can...

04 April 2019 4,777 2 View

I am planning for Native PAGE for a protein with pI 8.5 - 8.7. What should be the minimum difference of pH of buffer to avoid electrode switching?

The Gel has pH 8.9. and the pH of loading dye is 6.67. What should be the pH of running buffer to allow smooth running of my protein into the gel. I prepared buffer of pH 8.49 but I was unable to...

25 March 2019 9,877 6 View

Any suggestion what's going on?

I submitted a manuscript on a novel and interesting finding on July 30, 2018 to a reputed journal. Following editorial review it went to peer-review. It is over 8 months, no decision yet. What...

24 March 2019 9,737 2 View

How to find the natural frequency of fixed thick glass?

Dear researchers, i am looking for equation to find the natural frequency for fixed thick glass. please suggest

28 November 2018 2,736 2 View

Does any one know, how I can detect human blood from other animals in insect vector ?

Biochemical and Molecular methods please and If any a rapid test from forensic sector.

12 May 2015 6,612 3 View

What are the advantages growth of GaN on graphene in optoelectronics?

I would like to write my research plan for my PhD on this topic.

21 April 2015 4,301 3 View

Is it compulsory/mandatory to examine the mediating effect in the conceptual framework that was analyzed utilizing AMOS?

Increased value of invertebrates - Mediating variable

08 April 2015 2,834 5 View

How is oil content estimated in mustard seed? Can anyone provide me a protocol regarding this?

I want to estimate the percent of oil present in mustard seed.

24 March 2015 2,233 7 View

What is the minimum sample size needed to conduct the SEM analysis utilizing AMOS software?

05 February 2015 9,648 7 View

How to stop dye transfer from one cell to another?

I am trying to optimize ADCC for HIV infected cells. I am labeling my targets with CFSE and any other membrane or nuclear dye. My effectors are unlabeled. The problem is the second dye, no matter...

15 January 2014 7,731 3 View

How climate change will influences human and animal behavioral change?

The role of climate change influencing the human behavior as well as economics interest

01 June 2021 0 0 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

Do we need to write protocol for a second study of a systematic review that has been registered and published?

I have conducted and published a systematic review and meta-analysis research with the topic related to public health and health pomotion (protocol was registed in PROSPERO). Now we want to...

03 March 2021 8,920 3 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

1. What is the impact of having different scales in a survey? and how can we solve this problem before and after data collection (Literature)?

1. What is the impact of having different scales in a survey? and how can we solve this problem before and after data collection (Literature-based reflection)? Thank you for your time and for...

03 March 2021 2,870 3 View

What degree of missing data in a particular case/participant results in Expectation-Maximisation no longer working?

I'm dealing with a mediation model and am using the PROCESS module in SPSS. Due to SPSS and PROCESS being limited in the imputation methods - being unable to handle multiple imputation - the other...

02 March 2021 4,362 1 View

Do I need to do linear regression before running mediation analysis using PROCESS MACRO by Hayes ?

I just wanted to check if I need to run a linear regression separately if I am using PROCESS MACRO to run mediation analysis. Thank you.

02 March 2021 4,359 3 View