I have a set of confocal microscopy images. I am finding little difficult to conclude from the data, so need help from experts on this. I will upload the images as someone is interested.
No one will be able to answer the questions if there are no images. Put them up and start the convo!
Here are the files..I have uploaded the selected one for drugs..i can send you a full line as well.
https://drive.google.com/open?id=0B33EUVlZ3EyHZE1tNTRfU0tLYXc
Hi. Can you please expalin what you need help with? Are you trying to quantify the percentage of your cells which was affected with the drugs Or?
With more info I might be able to help.
Please provide information regarding your experimental setup, cell line used, antibodies used, conditions for each row of images, etc.
The experimental details are as follows
1. Cell line used HeLa cells
2. Cell lines were subjected to a concentration of 0.1ug/mL of drugs.
3. The images were taken after exposure time of 2h..
I do want to know the following facts
(a) can we quantify the cell death
(b) are they binding to the nucleus ?
(c) any other relevant information..whatever can be extracted otherwise.
Thanks
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