I have a set of confocal microscopy images. I am finding little difficult to conclude from the data, so need help from experts on this. I will upload the images as someone is interested.
No one will be able to answer the questions if there are no images. Put them up and start the convo!
Here are the files..I have uploaded the selected one for drugs..i can send you a full line as well.
https://drive.google.com/open?id=0B33EUVlZ3EyHZE1tNTRfU0tLYXc
Hi. Can you please expalin what you need help with? Are you trying to quantify the percentage of your cells which was affected with the drugs Or?
With more info I might be able to help.
Please provide information regarding your experimental setup, cell line used, antibodies used, conditions for each row of images, etc.
The experimental details are as follows
1. Cell line used HeLa cells
2. Cell lines were subjected to a concentration of 0.1ug/mL of drugs.
3. The images were taken after exposure time of 2h..
I do want to know the following facts
(a) can we quantify the cell death
(b) are they binding to the nucleus ?
(c) any other relevant information..whatever can be extracted otherwise.
Thanks
Its a ring opening reaction. I probably avoid overheating the mixture. But, I do need to remove acetic acid to run a column.
06 July 2017 4,455 3 View
syntax error is coming again and again in autodock vina
11 December 2016 1,535 9 View
I came across someone in recent days who is claiming to have solidified mercury by a novel method. While the stuff is being characterized, I wondered what has been done before and what is the...
03 April 2016 4,094 0 View
I have virus (viral hemorrhagic septicemia virus) in suspension and the experiment will not involve cells. What level of TCID50 is preferred?
11 August 2024 3,115 1 View
I am using Rhodamine6G as gain medium and silver nanoparticles as scatterers on a microscope slide and laser input 532 nm comes from above.
09 August 2024 9,894 2 View
I am experimenting with cancer and non-cancer cells in a 12-well plate for 4 days with a seeding density 1*10^4/well, however, I noticed that the control group growth rate slows down on D3. Should...
07 August 2024 2,283 2 View
Hello dear colleagues, We have prepared a manuscript on NiTi-based alloys and are seeking a second opinion on our current TEM results. If you are a Ph.D. holder with experience in TEM and have...
07 August 2024 9,563 0 View
After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...
05 August 2024 9,637 2 View
Are there any fluorescently labeled anti-Alpaca secondary antibodies raised in Donkey? So far I have only been able to find anti-Alpaca secondaries raised in Goat. Or is this not possible due to...
04 August 2024 4,255 1 View
Hello. I am working on ROS production of two systems: system A is cerium oxide and hydrogen peroxide, system B is cerium oxide nanoparticle, hydrogen peroxide and potassium bromide. I did some...
04 August 2024 5,974 3 View
average particle size calculation from TEM
04 August 2024 2,921 1 View
I am working on a SEM model where i have 7 latent variables (6 exogenous and 1 endogenous). In AMOS when I co-vary the exogenous constructs, only 2 paths are coming significant out of 6. But when...
03 August 2024 6,028 4 View
I work on MCF7 cell cell for anticaner purpose and I wa to do drug preperation the drug ( secondary metabolites extracted from Aspergillus) My question which solvent is better with these secodary...
03 August 2024 4,725 2 View