Mariam Adebayo

6 Questions 3 Answers 0 Followers

Questions related from Mariam Adebayo

Knockdown efficiency reflected in PCR but not western blot?

Hi, I am doing siRNA of a particular gene of interest. however, after several attempts, western blot is not showing evidence of this knockdown, I have tried different antibodies and also longer...

03 March 2019 4,535 4 View

What is the best method for quantifying phalloidin staining?

I did a phalloidin staining for f-actin, Is it possible to quantify the f-actin stretches between two different staining and how?

04 April 2018 9,071 0 View

Is it possible to do a Phalloidin staining for Actin on RFP transfected cells?

I am trying to stain for actin filament in RFP transfected cells which already show up at red. Is this possible and any ideas?

05 May 2016 5,680 2 View

What is the best way to seed cells on acrylamide coated coverslips?

i am trying to seed cells on coverslip coated with gels (acrylamide based hydrogels) My main obstacle is my cells aren't settling down as expected so they can grow instead they keep floating, or...

11 November 2015 994 4 View

Any advice on Ethidium Bromide and Calcenin staining for cells seeded on coverslip?

I am trying to stain cells seeded on hydrogel coverslip with EtBr and Calcenin in 2D culture. Any suggestion on the best protocol to follow would be greatly appreciated.

11 November 2015 5,507 3 View

Why does my citrate buffer turn milky during immunohistochemistry?

For my IHC protocol, I am staining for beta-catenin in ovarian sections during antigen retrieval. My samples are heated in citrate buffer for 45 mins. I have also read that signs to show that your...

12 December 2014 6,244 4 View