I am looking for a UAS fly line which has LAMP1-GFP , homozygous,in the 3rd chromosome. Where Can I get it?
(Transfection with two plasmids has worked for this cell line. pRL-TK(Renilla) is not getting transfected).
16 June 2024 6,687 2 View
If anybody has successfully done electroporation of RNA into HEK293T cell, kindly share the electroporation parameters. Or, if anyone is using Maxcyte STx machine for electroporation, please share...
28 January 2024 8,008 0 View
I have isolated plasmid and then linearised it with re. Then I put in-vitro transcription of the linearised plasmid but not band of mRNA could be observed only the linearised plasmid was seen....
19 November 2023 3,607 7 View
Hi I was wondering whether CMV and EF-1alpha promoters can be put in a single plasmid? Are there any compatibility issues between the two promoters? Thank you.
31 October 2023 9,919 2 View
What size of plasmid can be isolated from qiagen miniprep and midiprep kit, respectively? And at what temperature shall I grow a 36kb plasmid with 12.5ug/ml chloramphenicol resistance?
07 September 2023 2,600 3 View
I am trying to isolate BAC plasmid of size 36kb. I tried with Qiagen Miniprep kit and Zymo BAC purification kit and ran on 0.5% gel. The bands can be seen on the gel but no bands were after I did...
31 August 2023 2,932 4 View
I am looking for the ED50 of meropenem in mice against Klebsiella systemic/lung infection models. Is anybody aware of it? Thank you.
18 June 2023 2,018 0 View
Example: TEM reveals the layered stacks with different thicknesses
11 June 2023 7,653 3 View
Hi I am kind of looking to see if a comparative expression level analysis of the different Glycosylation enzymes of different CHO cell lines (DG44, DXB-11 and K1)is available in any publication. I...
12 April 2023 9,492 2 View
Provide arguments in favour of ur response, from field experience
05 April 2023 7,396 5 View
There are seven (7) established theories of macroevolution viz. i) Chromosomal speciation theory, ii) Shifting balance theory, iii) Punctuated equilibrium theory, iv) Allopatric speciation theory,...
10 July 2024 9,679 1 View
Hi ResearchGate community, how are you? I need to request your help to mitigate the generation of microbubbles detected during the slide preparation of sample metaphase chromosomal spreads. I...
01 July 2024 7,694 4 View
if the earth is spherical then rockets can use less energy to get to outer space by flying horizontally till they reach outer space or even they can nose dive and enter outer space from beneath...
17 June 2024 1,378 3 View
Silver staining process is used to stain this region, why?
12 June 2024 4,517 1 View
Contandriopoulos, Quézel et Zaffran claimed that both 2n=16 whereas 't Hart claimed tymphaeum 2n=14 tristriatum 2n=22, 2n=44
29 May 2024 3,952 1 View
Greetings! Has anyone used Dinitroaniline herbicides to double the chromosomes of haploid corn? If did, which one and the details, please. Thank you very much!
29 May 2024 9,836 1 View
I'm on a time crunch and have to fly back home and my return date to the lab where I'm doing my research is yet to be determined (hopefully in the next couple of months). I'm hoping I can store at...
15 May 2024 2,572 0 View
I was simulating 3 types of speed controller types for flying shears. P, PI and PD speed controller. And my question again is, what is the reason for not using PD control even if it can...
15 May 2024 4,806 3 View
Going futher, i am runing a nutrition experiment using black soldier fly meal (BSFM) and polychaete meal (PM) as a replacement for fishmeal targeting maturation in Litopenaeus vannamei. This is...
11 May 2024 3,985 0 View
The result from PlasmidFinder shows that the assembled genome harbored many plasmids. Consequently, I want to investigate whether the plasmids are encoded for ARGs. And determine if they are on...
08 May 2024 7,467 0 View