I need a good annotation software or tool for nucleotide and protein sequence? Genome is not sequenced and it is plant database.

More Gopal Dhedhi's questions See All

How to write a research paper?

please tell the research paper writing steps from beginning to end.

24 April 2024 978 5 View

How miRNA encoding genes are different from normal protein coding genes ?

As we know miRNA also encoded by some miRNA coding genes which is certainly different from protein coding genes, here i want to know how they differ from each other

12 April 2024 6,381 2 View

How to get Equilibrium for Lennard-Jones Potential?

Hi all! I want to get equilibrium for Lennard-Jones potential. How it will find and implement our system. please suggest any idea.

10 March 2024 7,525 1 View

Say, one has prepared bismuth nanoparticles using plant extract without the necessity of adding NaBH4, will there be Bi NPs or Bi2O3 NPs?

NPs= Nanoparticles NaBH4= Sodium Borohydride Bi= Bismuth Bi2O3= Bismuth Oxide A further question, without calcination. The person does not do any calcination.

10 March 2024 6,337 0 View

1. can we run isolated molecule (monomer dimer or trimer) as one unit only on gromacs?

Hi all, I want to run isolated molecules as one unit on gromacs. any idea how would i do that.

05 March 2024 2,096 2 View

Can we run MD without solvation?

Hello all, I had a question about MD, which I would appreciate if you could answer. I want to run MD without solvation. please suggest any idea.

05 March 2024 5,386 2 View

Choice of enzymes for glycopeptide analysis in plasma/serum sample - Lys-C followed by Trypsin vs Trypsin/Lys-C mix ?

To study proteomics or glycoproteomics in biological samples, we always go for Lys-C and followed by trypsin digestion. Promega & others offer Trypsin/Lys-C mix. What is the efficiency of using...

13 February 2024 7,027 0 View

How does polarity affect welding? Whast is the arc physics (in detail) behind this phenomenon?

I am getting conflicting information from the web. Some say it is one way for arc welding and another way for tig welding and mig welding. I need some clarity as to how arc physics works. There is...

05 February 2024 3,017 2 View

What is my sds page telling about the deglycosylation?

Hi all, I used pngase f to release glycan from transferrin. here is my protocol. 1. I prepared storage buffer for the pngase f without glycerol and aliquot them into different vial 2. I took 10...

21 October 2023 3,658 7 View

Could microplastics in soil introduce drug-resistant superbugs to the food supply?

Any one can explain about this

25 August 2023 5,617 1 View

How can I prepare virus for a TEM or SEM imaging?

I have virus (viral hemorrhagic septicemia virus) in suspension and the experiment will not involve cells. What level of TCID50 is preferred?

11 August 2024 3,115 1 View

How to learn more about SPSS and its Application?

I would like to learn more about SPSS and Its application especially in regards to data analysis. Please suggest me how I can learn more about it. Thank you so much.

11 August 2024 9,101 4 View

Can I base on reverse DNA sequences to perform alignment, convert to amino acids and GenBank submission?

I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?

11 August 2024 5,138 1 View

Baseline drift in HPLC? What causes this?

Hello, Why do i see this baseline drift when i compare my blank (black) to the sample (blue)? Any suggestions as to why this happened? Thank you!

11 August 2024 3,770 4 View

Text-Communication from the M1 Hand Area using BCI—and then there is Elon Musk?

Willett, Shenoy et al. (2021) have developed a brain computer interface (BCI) that used neural signal collected from the hand area of the motor cortex (area M1) of a paralyzed patient. The...

10 August 2024 7,180 0 View

Handling Missing Data and Building a Predictive Model with Incomplete Information ?

I am developing a predictive model for a water supply network that involves 20 influencing points. However, I only have historical data for 10 out of these 20 points. I would like to know how to...

10 August 2024 4,005 2 View

Has anyone applied Python in the field of textile engineering for data analysis, automation, or smart textiles?

I'm currently exploring the application of Python in textile engineering, specifically in areas like data analysis, process automation, and the development of smart textiles. I'm interested in...

10 August 2024 7,429 2 View

Which Scopus Journal provides the most affordable fees?

"PUBLISHING IN A SCOPUS JOURNAL" Researchers are now at a cross road. The critical need to publish in a Scopus or ISI, etc journal is ever vital. Journal Publication fees must be submitted....

10 August 2024 8,621 1 View

Seeking Advice on Viability and Execution of Undergraduate Thesis Topic?

Hello everyone, I am currently developing a thesis proposal and would appreciate your input on its viability and how to effectively carry it out. My proposed topic is: "Does the perceived threat...

10 August 2024 8,992 0 View

How can I use the cif data obtained from rietveld refinement extracted via gsas2, for microstructural analysis using ETEX software?

09 August 2024 7,718 0 View

Nagesh Kuravadi

You can assemble your transcripts(cDNA) and annotate using regular annotation pipline like

MAKER : http://www.yandell-lab.org/software/maker.html

annot8r : http://www.nematodes.org/bioinformatics/annot8r/

BLAST2GO :http://www.blast2go.com/b2ghome

Shishir K Gupta

Hi Gopal, you can proceed with offline Augustus .