I have transmittance from the photospectrometer. How to find out the the lysozyme activity?

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William Letter

This is based on a classic paper and method. Biochim Biophys Acta. 1952 Mar;8(3):302-9. The measurement of lysozyme activity and the ultra-violet inactivation of lysozyme. SHUGAR D.

Nauman Nazir

Respected @Bill Letter,

Thanku for your precious time.

Sir I have tried to find the full text of Shugar D., but I couldn't.

I have found some formulae at Worthington Manual, but it is not helping either. it will be a great favour if you could help in letting me know the formulae.

REGARDS,

A keyword search on the web will provide you with exactly what you are looking for. A few examples of related documents are linked below.

http://www.worthington-biochem.com/ly/assay.html

http://www.sigmaaldrich.com/technical-documents/protocols/biology/enzymatic-assay-of-lysozyme.html

Thanku so much for your time again.

I have found it helping. But more then helping, I found your answers encouraging.

I am calculating the activity and I will share it with you as soon as I do so.

Regards,

You are very welcome. Good luck to you with your project.