26 September 2023 1 8K Report

Burkholderia glumae cause panicle blight of rice. Please share Burkholderia glumae colonies characteristics and colour

More Hajra Azeem's questions See All
If I have adjusted OR for the group of interest, but a paper does not provide it for the control group, how can I calculate the ES in the control?

Sentence in question, "The rate of SCD was higher among PHIV versus non-HIV-infected patients (21% vs. 6.4%; p 

29 March 2024 1,563 0 View

Any one who is working on Sping 1/2 antiferromagnets?

I am looking for someone expert in spin 1/2 antiferromagnetic systems. I have a manuscript ready for the publication but need an expert reviewer. I will acknowledge the reviewer as co author. Thanks

04 March 2024 1,474 0 View

How does the crystallite size (calculated from XRD) impact the performance of electrochemical CO2 reduction?

The lower crystallite size of the electrode material will contribute to higher catalytic activity and selectivity?

20 October 2023 7,062 1 View

Why shelf life of bread is not going to increase upto one month even though every 4th researcher is doing research on bread?

Answar?

31 August 2023 5,929 4 View

Can someone share protocol Osteoclast differentiation from RAW264.7 and TRAP staining??

Hello Everyone!!! I am trying to differentiate RAW264.7 cells (1million cells/1ml/ well of 12well plate) using 10ng/ml or 50ng/ml of RANKL concentration for 5 to 7 days. Later on the TRAP staining...

10 July 2023 4,040 1 View

I am interested in collaboration with anyone who is expert in Reitveld refinement?

I am looking for someone who can perform above mentioned analysis for me. I will offer him/her to be a coauthor of my upcoming publication. Thanks

17 June 2023 4,565 0 View

Is it permissible to conduct an individual participant data meta-analysis on case reports / case series?

I am attempting to conduct a SR with a meta-analysis on a cardiology condition that is not often reported. There are a few retrospective studies, but many more case reports / case series. Based...

11 December 2022 7,657 4 View

Why EVs batteries have relatively lower voltage?

High battery voltage eliminates the requirement for a bidirectional DC/DC converter. If the battery voltage is high enough, the requirements of the separate DC/DC have vanished that further...

13 November 2022 8,861 1 View

Could you please recommend some crops or grasses or vegetables ?

that can sustain Higher temperature drought conditions sandy soil saline water please recommend crops for different seasons like winter, summer, spring and autumn.

03 November 2022 155 8 View

Reitvelved refinement using Fullprof?

I am frustrated by Fullprof. I aim to refine structural parameters by Reitveld method using Fullprof and have watched dozens of tutorials on youtube. However, either I get an error related to...

29 October 2022 2,855 6 View

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Pink bacterial colonies?

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My rosetta strain has chloramp resistance and i am transforming my plasmid with amp resistance but not getting colonies on chloramp/amp LB plates?

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Has anyone calculated the effective concentration at 50% (EC50) value of different fungicides for the fungus Tilletia horrida?

How can we measure their colony diameter?

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I have constucted a plasmid vector from puc19 with no antibiotic and lacz gene. The problem is screening for transformed cells. Any suggestions?

Get too many colonies, which are mostly non-transformants when tested by colony PCR

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I am not getting proper colonies while ligating one of fragments in my vector the colonies seems to be normal but in light it seems lysed any reason?

I am working on cloning of a gene fragment inside my vector everything we tried to do correctly like making insert and vector with utmost care but after ligation the morphology of colonies seems...

11 July 2024 9,010 3 View

Can someone provide any tips for cell quantification on Fiji, ImageJ?

Hi. I have brightfield images of stem cells. I need to count the total number of cells/image. Some cells are individual and some cells are in colonies. Using average cross-sectional area of...

05 July 2024 4,197 1 View

Cloning restrction digestion needed before sequencing?

Hello, recently i am an issue swith cloning where I am using the subclone method to insert my gene length is 1.5kb and my vector(pMAL-c6T) is 5kb I got the colonies which i screened through the...

04 July 2024 7,977 2 View

Why I am unable to get the expected insert at the cloning site?

I am trying to clone a gRNA (23 bases) into the Crispr cas9 pDirect23A vector by using restriction enzyme cloning. I have performed a complete experiment, starting with plasmid isolation,...

02 July 2024 5,448 2 View

Why my transformed colonies do not carry my insert?

I digest my plasmid with KpnI-NotI. I cut the expected 4kb band from the gel, purified it and then I did ligation [1:3 and 1:5 vector:insert ratio] and transformation. I found 18 transformed...

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