01 March 2016 7 9K Report

I have questions a bout remote sensing of oil pollution of the soil,does anyone have any information that can help me?i would really appreciate for any help.

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Can I use sonicator only to lyse the exosomes and measure their protein contents using BCA assay kit?

Hello, I want to ask regarding exosome protein quantification method. Is it necessary to lyse exosomes before measuring their protein contents using BCA assay kit? Or can I just straight away...

23 July 2024 4,603 4 View

What are the recent advances in aromatic plant research?

looking for areas of research

20 April 2024 8,565 1 View

What factors should be considered in developing a reliable serological test for HDV antibodies that can effectively account for the diverse genotypes?

Prior to the research carried out by the research group who published this journal, they assessed HDV RNA detection's diagnostic efficacy utilizing the HDV RNA detection technique and discovered...

11 April 2024 1,849 1 View

Why my unstained, isotype (IgG) and positive samples (U87 cells) have same histogram results?

Hi, I just want to ask regarding flow cytometry method for indirect staining of U87 cells (Glioblastoma) as I'm still quite new in using this method. My objective is to observe the expression of...

23 March 2024 8,883 1 View

How should I be dissociating 30k embryonic epithelial cells for FACS to prevent clumping?

The project I'm working on involves dissecting out a small piece of epithelial tissue that we're going to run through FACS and single cell RNA seq. My usual way of dissociating tissue is in 50ul...

24 June 2023 9,688 0 View

Lysotracker bleaching and toxicity (with movie!)?

Hi I'm using LysoBrite (a variant of Lysotracker) for live cell imaging with iPSC derived neurons. The problem I am having is that it bleaches fairly rapidly (within ~1 minute of imaging) and the...

08 June 2021 2,762 0 View

How can I analyze qPCR data without control sample?

I want to quantify a few genes expression in two different samples. I have no control sample. How can I analyze my qPCR data if I want to use the delta delta CT method? Would it be more suitable...

19 May 2021 6,745 3 View

Is cell culture contamination always obvious?

I understand Mycoplasma can fly under the radar, but are other forms of contamination obvious over extended culture? I have been growing neurons for 30 days, and since there are no obvious...

05 March 2021 4,304 4 View

Live Cell Imaging Media Formulation?

Hi, I'm doing some live cell imaging experiments with SHSY5Y cells. Could someone pass me their media formulation?

22 September 2020 9,123 3 View

Is perfusion neccessary for brain IHC analysis?

Hi, we are generating primary neurons - I was curious if the remainder of the brain could be fixed in PFA and used for IHC analysis without prior perfusion fixation?

21 September 2020 4,462 3 View

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