Hi, I have purified MBP fusion protein, is it possible that i could check in gel actvity of my protein while the taq is still attached if it doesnt interfers.
1. Protein -GQFYLNE-LINKER -6His OR 2. Protein-ENLYFQG-linker-6His Further is this linker sequence GGGS ok to go with
07 August 2018 7,493 0 View
I want to separate my plant protein sample via gel filtration prior to affinity chromatography; please guide
02 March 2018 2,044 9 View
I have stored my leave sample at -80 and want to prepare a soluable fraction for protein purification. I want to ask is grinding it in liquid nitrogen and further dissolving it in chilled buffer...
11 December 2017 8,126 3 View
untagged protein purification
05 June 2016 1,812 4 View
Hi, I am trying to purify my POI its copurying with a chaperon. so i have decided to do a MgATP wash to get rid of chaperon. But my query is that i am using pottasium phosphate buffer at Ph 7.4...
01 January 1970 9,709 1 View
I got these smeared bands quite often lately. We typically run the gel at 140V with a 10-12% gel and do a wet transfer at 220 mA for 1.5 hr in cold room. We also noticed some dirty spots/dots (see...
10 August 2024 7,480 3 View
I am staining some brain sections stored in cryoprotectant that express a Histone H2B- GFP fusion protein that were generated ~10 years ago. I know I need to enhance signal with an anti-GFP...
07 August 2024 5,338 2 View
I have been working on Red blood cell-derived extracellular vesicles as Antisense Oligonucleotide (ASO) carriers. We normally run agarose gel to quantify the loading efficiency. I used naked ASO...
06 August 2024 3,130 2 View
I am having an issue with my gel image where my PCR product is not appearing very bright on the gel. When I perform gel extraction, the A260/280 purity value is very low. I used the Qiagen gel...
05 August 2024 9,798 3 View
Hello everyone, I performed a PCR yesterday, and the results showed no bands on the gel. Of course, I probably missed some crucial steps, like adding my samples to the PCR strips themselves, for...
31 July 2024 2,406 6 View
We are working on biopolymeric hydrogels. Our system is highly viscous and sticky, and the gel formed are high in strength. We are unable to use pH electrode and pH strip. Please suggest an easy...
30 July 2024 942 2 View
I am using CuBr/THPTA for a click reaction in total cell lysates. I am facing issues with my protein sample in non-reducing SDS-PAGE where it's not migrating properly and most of it remains at the...
29 July 2024 950 4 View
I found that several expression constructs of published protein structures containing an N-teiminal fusion tag GAMGSGIQRPTST. What's the founction of this fusion tag?
23 July 2024 1,353 4 View
I am performing IgG purification and I have to show my results on SDS-PAGE. I use 10% tris glycine gel and prepare the samples under non reducing conditions. I am new to antibodies and therefore...
23 July 2024 6,664 6 View
Hello, I was running a 12% SDS Page electrophoresis on few granulosa cell samples and got this result after the ponceau staining. The total protein lysate seem to aggregate at 70 kDa ladder mark...
21 July 2024 5,128 4 View