1. Protein -GQFYLNE-LINKER -6His
OR
2. Protein-ENLYFQG-linker-6His
Further is this linker sequence GGGS ok to go with
Hi, I have purified MBP fusion protein, is it possible that i could check in gel actvity of my protein while the taq is still attached if it doesnt interfers.
10 November 2018 1,627 0 View
I want to separate my plant protein sample via gel filtration prior to affinity chromatography; please guide
02 March 2018 2,044 9 View
I have stored my leave sample at -80 and want to prepare a soluable fraction for protein purification. I want to ask is grinding it in liquid nitrogen and further dissolving it in chilled buffer...
11 December 2017 8,126 3 View
untagged protein purification
05 June 2016 1,812 4 View
Hi, I am trying to purify my POI its copurying with a chaperon. so i have decided to do a MgATP wash to get rid of chaperon. But my query is that i am using pottasium phosphate buffer at Ph 7.4...
01 January 1970 9,709 1 View
The first pdf file I uploaded had an error. So I uploaded an updated, corrected pdf of that paper with a different pdf name. I dpon't want the old copy to be download or read.
07 August 2024 9,508 1 View
I'm trying to calculate the tolerance factors of the hydride perovskite and the value calculated by me and the reported values are contradictory. Need more clarity in using the correct ionic radii...
01 August 2024 2,614 1 View
How to change the displayed full article text to its corrected version? In the file on the page of the journal where I published the article, there was an error in the text, the table is...
30 July 2024 3,229 2 View
The entropy measured of molecular graphs plays a crucial rule. The network structures in some cases are very lengthy calculations to handle. Some author avoid to construct table where as most...
30 July 2024 3,126 0 View
I am writing to request a correction regarding the affiliation of one of my co-authors, Donald Ho. Currently, the author's profile incorrectly lists an affiliation with The Hong Kong Polytechnic...
28 July 2024 6,824 0 View
I need to know the approximate size of my target protein for Negative-stain EM. I have an alpha-fold predicted structure of the protein. I do not see any turtorial on internet for estimating the...
21 July 2024 5,732 3 View
Dear sir/Madam, I am Parinaz Parsa the first writer of article "Optimisation of probiotic yoghurt production enriched with phytosterols" and understand that one person is mentioned as writer which...
14 July 2024 9,579 2 View
I am trying to configure the instrument in Profex but I can't get a chi-squared value below 9.65 for LaB6. This is the configuration of the equipment, which is a Bruker D8 Advance with a Linxeye...
14 July 2024 4,767 1 View
In My article: The Extended Pedersen hypothesis (published, February 1988 Clinical Physiology and Biochemistry 6(2):68-73,) there is mistake of the name of first Author: it is not Catherine...
11 July 2024 1,801 2 View
I am having trouble expressing a protein and have heard that co-transforming it can improve stability and increase expression, so I am looking to try this experiment. I only need the kinase domain...
08 July 2024 2,935 5 View