1. Protein -GQFYLNE-LINKER -6His
OR
2. Protein-ENLYFQG-linker-6His
Further is this linker sequence GGGS ok to go with
Hi, I have purified MBP fusion protein, is it possible that i could check in gel actvity of my protein while the taq is still attached if it doesnt interfers.
10 November 2018 1,537 0 View
I want to separate my plant protein sample via gel filtration prior to affinity chromatography; please guide
02 March 2018 1,948 9 View
I have stored my leave sample at -80 and want to prepare a soluable fraction for protein purification. I want to ask is grinding it in liquid nitrogen and further dissolving it in chilled buffer...
11 December 2017 8,013 3 View
untagged protein purification
05 June 2016 1,728 4 View
Hi, I am trying to purify my POI its copurying with a chaperon. so i have decided to do a MgATP wash to get rid of chaperon. But my query is that i am using pottasium phosphate buffer at Ph 7.4...
01 January 1970 9,624 1 View
Dear Researchers I am trying to perform a PIL simulation using STM32F4 Discovery board and comunication serial USB TO TTL. During simulation I receive the following timeout error: An error...
01 March 2021 2,327 1 View
I am trying to find how to know the density needed for a pva electrospinning in a 0.6mm neddle, the electrospinning will be from top to bottom and the problem I really have is the solution because...
25 February 2021 2,615 3 View
I would like to make a correction in a co-author in your paper which is wrong. This name is also in other papers of Kenton Ko. Zey Ko is not a Co-author . The correct name is Zdenka...
24 February 2021 3,476 4 View
Hello, I have run into an issue that has sparkled a debate at work: is the LLOQ/ULOQ impacted by injection volume under the following circumstances: 1) Same dilution factor of all samples 2)...
23 February 2021 9,159 3 View
I am trying with a Unet code ontained from net ...in tensorflow. When trying to predict the segmentation result with the trained and saved model with metric " iou" using "CustomObjectScope"...
22 February 2021 5,254 1 View
I was wondering if anyone knew how to perform the Haldane correction for SPSS (this is the definition of the correction -...
21 February 2021 2,882 1 View
hi everyone I am simulating a unit cell structure in HFSS using Flouquet port having two modes for absorption I have used this formula:- absorption=...
17 February 2021 9,178 2 View
I would like to calculate the magnetic force on a Fe304 particle which is into a liquid solution . The magnet is above the solution , and there is no electricity. I found this formula : F = (V...
13 February 2021 4,770 3 View
I am working on Taylor Couette flow between concentric cylinders and the results are correct for the full case. however, I wanted to do symmetry case to improve the mesh. I have tried periodic...
11 February 2021 2,193 6 View
UACI - Unified Averaged Changed Intensity between cipher images. Why some papers refer ideal value to be 33.33 and some refer it to be 33.46? Which is the correct value? (In reference to image...
11 February 2021 9,562 2 View