Tehseen Rubbab

6 Questions 10 Answers 0 Followers

Questions related from Tehseen Rubbab

I have purified MBPfxafusion protein, is it possible that the fused protein can still show ingel activity?

Hi, I have purified MBP fusion protein, is it possible that i could check in gel actvity of my protein while the taq is still attached if it doesnt interfers.

11 November 2018 1,584 0 View

Hi what will be the correct orientation to attach TEV protease to protein ?

1. Protein -GQFYLNE-LINKER -6His OR 2. Protein-ENLYFQG-linker-6His Further is this linker sequence GGGS ok to go with

08 August 2018 7,465 0 View

How to pack sephadex G-75 column please guide ; i need to separate my protein sample via gel filtration ?

I want to separate my plant protein sample via gel filtration prior to affinity chromatography; please guide

03 March 2018 2,000 9 View

How to extract active protein from leave sample, so that its SOD activity is retained during the procedure?

I have stored my leave sample at -80 and want to prepare a soluable fraction for protein purification. I want to ask is grinding it in liquid nitrogen and further dissolving it in chilled buffer...

12 December 2017 8,084 3 View

What is the most efficient way to isolate & purify untagged protein from transformed plant?

untagged protein purification

06 June 2016 1,780 4 View

Buffer to use with talon resin for MgATP wash to remove chaperons

Hi, I am trying to purify my POI its copurying with a chaperon. so i have decided to do a MgATP wash to get rid of chaperon. But my query is that i am using pottasium phosphate buffer at Ph 7.4...

01 January 1970 9,675 1 View