I have plotted the ELISA standard value using sigma plot. How could I know the unknown protein concnen using the absorbance in sigma plot ?

03 February 2025 1 9K Report

I have attached the file of unknown protein Absorbance and standard plot value.

In this case, I would use a linear fit to the standard curve, Y=mX+b, where Y is absorbance and X is protein concentration, m is slope and b is y-intercept. Then you can calculate the concentration from the equation X=(Y-b)/m.

The exponential equation may fit the standards a little better, but not much.

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