Hi, give it try on culture media test on the lab to check its efficacy on the pathogen , mix the oil with media culture with different concentration like 1,2,4,6% and check the inhibition effect on the mycelia.

good luck

Mixing oil with melted agar, once dried inoculate it with a fragmen of a new culture of the pathogen

You can use wells method, prepare your culture media (PDA) and pour it on a petri dish. Use cork borer to make holes on the media and the add different concentration of your oil i.e. 2%,4%,6%,8%. Used a synthetic fungicide as a positive control and water as a negative control in different holes made on the media and then inoculate the media with the fungus. Measure and the record the zone of inhibition with respect to each treatment and don't forget to replicate them. All the best

Can make a combination between agar and oil used in different ratios

DOI: 10.1080/09583157.2017.1281380

Methilated rape oil (commercial name Toil) at a concentration of 1.5% together with some registrated active ingredients acts as insecticides against the overwintering forms of San Jose scales, aphids and mites eggs in fruit growing.

Soybean edible oil at a concentration of 2% is used in organic apple production systems in management of apple powdery mildew. All The Best, C.

Dear Phumelela Peace Mwelasi , You have not mentioned where do you want to evaluated means either in field or in Lab. The concentrations will be adjusted according to Lab/field evaluation.

In the Lab you should use minute concentration but for field your concentrations must be higher as compared to Lab and Greenhouse experiments.

The experiment will start in the lab first at Muhammad Rizwan Bashir Thank for your answer.