The between group factor is "diagnosis at 19" with three levels: Controls, AUD and ED
The within group factor is time at diagnosis with two levels: 14 and 19
- I would preferably carry out this data analysis in graphpad prism
If your two variables are considered equal in their effects ,you can choose factorial , otherwise you need to go to a split plot with RCBD or CRD as you choose. Cheers.
I do agree with Prof Dr Medhat Elsahookie.
If the two factors possess the same importance, you could use Factorial with RCBD.
However, I advise you to use Nested design.
The correct design is factorial and the analysis approach is two-way ANOVA, where the main effects of the two factors and their interaction (combined effects) are considered.
ANOVA always takes categorical independent variables and continuous responses (dependent variables). However, it is important to also note the underlying assumptions of ANOVA: Normality of distribution of the responses and also uniformity of variances (uniform variability). I hope this help.
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