Hi,
I'm planning on doing oligodendrocyte staining on frozen and paraffin brain sections of postnatal (up to 2 weeks old) and adult mice.
I was wondering which antibody would you recommend for cytoplasmic staining of oligodendrocytes. Unfortunately, my other marker is nuclear, and would therefore preferer a cytoplasmic or membrane marker.
I know that there are a few commercial antibodies, however, I can't deicide which one would give me the best signal to clearly distinguish oligodendrocytes from other cell types in the CNS.
Thank you for your suggestions,
All the best
Fran
Hello, Fran!
Article Immunohistochemical Markers for Quantitative Studies of Neur...
Oligodendrocytes and Oligodendrocyte Precursor Cells
Eight different antibodies labeling oligodendrocytes, myelin-antigens, and oligodendrocyte precursor cells were evaluated (Table 5). In the adult neocortex only, the antibody against CNPase and the anti-oligodendroglia antibody (RIP), also recognizing an epitope in CNPase (Watanabe et al. 2006), labeled the cell bodies and the myelinated fibers (Figure 3E, shown for CNPase only). The staining for MBP labeled the myelinated fibers, and in contrast to the staining for CNPase, MBP+ myelinated fibers were still detected in tissue fixed in formalin for 10 years (Table 5). In agreement with recent studies locating the P25α-antigen/tubulin polymerization promoting protein (p25α) to oligodendrocytes (Skjoerringe et al. 2006), the anti-p25α antibody labeled round cell bodies with the nuclear morphology and distribution of oligodendrocytes but not myelinated fibers (Figure 3F). Staining for O4-sulfatide resulted in an inconsistent, weak staining of myelinated fibers and pyramidal neurons in adult tissues when applied to cryostat and paraffin sections (data not shown). Antibodies against Nkx-2.2 and platelet-derived growth factor-α receptor (PDGFα-R) expressed by oligodendrocyte precursor cells, labeled small cells with round nuclei in the intermediate zone in fetal tissue (Figures 2G and and2I)2I) and subcortical white matter from infant brain (Figures 2H and and2J);2J); only very few cells were shown in adult brain (data not shown). Despite the use of different protocols of staining and antigen retrieval, and the additional use of cryostat sections, we were unable to show staining for NG2 (Table 5).
- Badges
- Science topic