In NCBI gene Bank, only partial sequence is available. Is it correct to calculate the molecular weight size based on publicly available sequence of this particular species?
Collaboration is essential for ensuring learning for all learners in one classroom. Blackboard collaboration tools like blogs, wikis can be considered the best platform for increasing interaction...
17 February 2020 2,991 3 View
I do PCR reaction for my gene (it's 413bp). my pcr bands are so weak. I examined different template concentration (template is FMDV cDNA, 4900 nt),increase Mgcl2 concentration, and reduce primeres...
04 December 2018 4,772 15 View
During the culture of granulosa cell I found that cells are in cluster. So can anyone please suggest me how can these cluster of cells be separated out ?
21 June 2018 1,376 5 View
13 February 2016 8,286 10 View
01 January 2016 7,340 3 View
05 March 2015 8,961 4 View
To my knowledge, there is no solid correlation between temporal or spatial coherence and the polarization of light (linear, circular or elliptical) because the coherence arises from the nature of...
04 January 2015 3,902 12 View
I am Interested in making Fluorapatite.
24 August 2014 3,948 8 View
I am using cell signalling primary antibody and secondary antibody from sigma and substrate is NBT/BCIP. primary Ab dilution 1:1000 (3hrs rocking) sec Ab 1:10000 (45min rocking) primary ab...
14 March 2014 7,713 5 View
I want to add the XXX journal output style in endnote web. In my endnote web only 21 output style are available, e.g. numbered, Ris, science, etc.
09 March 2014 1,536 11 View
How to calculate the RMSD values for a MD simulation using MOE?
07 August 2021 0 0 View
When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.
16 June 2021 0 0 View
Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...
03 March 2021 5,978 5 View
Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...
02 March 2021 4,569 4 View
I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...
01 March 2021 8,544 1 View
Hi In Molecular Dynamics Simulation, how do I make changes to the force field according to my protein? I got this error in the add-ion stage: Fatal error "./ amber99sb-ildn-HHH.ff / force...
01 March 2021 8,747 4 View