Why tick's salivary gland extract didn't run in the gel (SDS PAGE )??

Greetings, I want to make SDS PAGE for my sample (Tick salivery gland extract), I tried concentrations 10%, 12%. I also tried high molecular weight ladder. But the protein stopped at the top and...

25 February 2021 7,698 3 View

What really is difference between high cycle and low cycle fatigue analysis in abaqus?

I have question regarding capabilities of abaqus for high cycle fatigue? Why do we need high cycle fatigue? Also, as abaqus only supports elastic material properties for low cycle fatigue, what is...

18 August 2020 6,411 5 View

Abaqus tyre rolling simulation?

I am currently running tire rolling simulation using dynamic explicit analysis in Abaqus. I get a vertical acceleration of very high magnitude(about 100 times more). What is the best practice?...

03 March 2020 979 3 View

VCCT with contacts ansys apdl?

I have a question regarding contacts use with VCCT in ansys apdl. I realized that under compression, the there is interelement penetration. When I used contacts with VCCT, I get non-convergent...

31 July 2019 1,713 1 View

What are the metrics for assessing the tipping points for a hydrological system?

What are the metrics for assessing the tipping points for a hydrological systems? I know they look appearances and disappearances of species for ecological systems but just wondering if there are...

28 October 2018 3,678 5 View

Fatigue crack growth in abaqus?

Dear researchers, Has anyone used abaqus for fracture crack growth using XFEM. I wanted to ask if the fracture crack growth criterian as fatigue is available in abaqus 2019 version. Because I...

01 January 1970 9,050 3 View

How to analyze a particular region of my simulation box in GROMACS?

Hi, I am using GROMACS 5.1.4 to simulate water in contact with a surface. I am only interested to calculate some properties of water near the surface. Let's say at a distance of x nm from the...

03 March 2021 9,989 2 View

How to get onecolumn document from standard IEEE ACCESS LateX template?

I have prepared a manuscript in IEEE Latex template, but for some purpose, I need to prepare a word file as well (detailed format is not required). Compared to two column .tex file, one column...

02 March 2021 1,830 3 View

How can the modification of garden soil cause global warming?

Hello, I am working on a soil project and I found a primary research article that tests how the modification of agricultural soil can cause global warming, using a thermal collector, however I...

02 March 2021 7,161 3 View

Does anyone know how to plot my station to a section plot on Ocean Data View (ODV)?

I made a vertical section plot with WOA .nc file on ODV, and now I want to plot my stations (csv. file) in it. Does anyone know how to import my points to the section? They are two different...

01 March 2021 3,610 1 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

How to address this issue in the analysis of Real-Time PCR results?

To dear Researchers, I was analyzing a series of concentration for estimation of Real-Time PCR efficiency. The concentration was 1:10. I used MS-excel to evaluate Slope. The result of slope was -8...

01 March 2021 8,683 4 View

Does anyone know to clarify these changes?

01 March 2021 1,290 2 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

Can I download BibExcel for MacOs?

Hi everyone, I would like to ask how I can download BibExcel software for MacOs? There are some links which are supported by Windows but I could not find it for Mac. or is there any other software...

01 March 2021 652 1 View