I had weak PCR bands for Adeno virus and when I did a nested PCR using the same primer and the pcr products I got no bands, what is my mistake?

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Sabine Strehl

How much of your first PCR product did you use as input for the second round?

Douglas Mcintosh

The original PCR product may have been a non-specific band (with the same molecular weight as the "true" amplicon. That being the case, the second pair of primers would be unable to bind to the false amplicon and no band would be seen.

Thibault G Sana

Agree with Douglas, you should sequence your PCR product to make sure it is specific, or not.

Good luck,

Thibault

Fletcher Del Valle

Thank you everyone for your advise, I really appreciate it.