H4 cells with 100000 cells per well
Mengjing Zhang
Dear Prof, is there a subtle distinction between the terms "substitution" and "doping" when referring to modifying ceramic dielectric materials for example Na0.5Bi0.5TiO3? Is there any difference...
08 July 2024 2,043 3 View
Hi to all of you! My goal is to create synthetic silica beads. These beads ought to have a specified size. Could someone please advise on how to proceed or give me the articles I need to finish my...
02 July 2024 8,170 1 View
Handbook [of] Heat Exchanger Fouling: Mitigation and Cleaning Technologies Hans Müller-Steinhagen IChemE, 2000 - Technology & Engineering - 382 pages
29 June 2024 8,259 0 View
Is it possible to achieve this by combining flux tally and reaction rate cards?
24 June 2024 3,727 3 View
As Clive Humby said, "Data is the new oil." With advancements in data analysis, we can now re-analyze previous datasets. Data gathering is expensive and time-consuming, but some datasets can be...
20 June 2024 6,418 2 View
Im currently writing my Thesis on Challenges/ Problems/ Particularities regarding Dark Tourism and have a little trouble finding the right literature. I have found a lot of literature for dark...
14 June 2024 7,262 2 View
When IC50 cannot be obtained according to the cell viability chart, what should be the dose selection under cell culture conditions? Would it be beneficial to go with sham control? For example,...
11 June 2024 9,109 0 View
Y=0.0011x+0.1031 (Standard curve eq) Chamomile sample 20mg/mL Absorbance(y)= 1.16 x=960.82(µg/mL) Chamomile sample 10mg/mL Absorbance(y)= 0.80 x=633.55(µg/mL) Tridax sample...
01 June 2024 7,843 0 View
This question aims to explore the comparative effectiveness of using a patient's own tissue (autologous) versus synthetic materials (alloplastic) in facial reconstructive procedures, focusing on...
25 May 2024 1,791 0 View
Dear researchers, I have recently synthesised MXene by etching MAX phase source using in-situ fluoride based etchant and collected the supernatant solution obtained after the washing process. My...
22 May 2024 5,269 2 View
I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?
11 August 2024 5,138 1 View
After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...
08 August 2024 1,668 3 View
I'm trying to find a DNA extraction method for fungi that does not require equipment and heating. Is there anyone who can suggest an alternative option? Thank you
08 August 2024 4,733 2 View
I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...
05 August 2024 9,059 3 View
I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...
04 August 2024 501 2 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
I have been attempting to extract DNA from Bacterial, Fungal and Yeast banked samples (>1e7 cells) using Prepman Ultra reagent and I seem to be struggling to obtain a sequence. Although the...
01 August 2024 2,079 0 View
I am currently working on a project involving liposomes and need to determine the maximum volume of siRNA that can be added to a 2.5 mL liposome solution with a total lipid concentration of 10...
30 July 2024 6,420 1 View
I am attempting to isolate picocyanobacteria from seawater (pre-filtered with a GF/D membrane) using the pour plate technique and filter plating method (Kearney et al., 2022) on Pro99 and SNAX...
30 July 2024 1,040 1 View
It has been documented that apoptotic cells themselves can induce phosphorylation of serine 139 on H2AX (γH2AX) due to DNA fragmentation during apoptosis (doi: 10.1074/jbc.275.13.9390). As γH2AX...
28 July 2024 7,983 2 View