I co-stained the GFAP and D-serine in the frozen slice but could not find the colocalization of these two markers?

24 August 2016 1 1K Report

I co-stained the GFAP and D-serine in the frozen slice but could not find the colocalization of these two markers.In fact, the astrocytes are able to produce D-serine, Why? In addition, I detected the colocalization of NeuN and D-serine antibodies.

Rafael Franco

I cannot figure out why you should "see" colocalization even if D-serine is produced by astrocytes.

In any case be aware that you need a positive control.

Also I wonder where you see the D-serine labeling in NeuN stained cells. In the nucleus perhaps? This is the second concern, some degree of unspecificity for anti D-serine antibodies.

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