How can I select the best plasmid after sequencing?

I have transformed a drought-related gene of 1.7kb into pGEMt vector (E.coli DH5 alpha strain). After sequencing the plasmids (3 replicates) 4 changed amino acids are visible at different...

06 February 2021 336 3 View

What can be the problem in the isolation of Plasmid?

I've tried transformation of a 1.7 kb gene with DH5 alpha strain of competent cells and also used PuC19 as a control. After selecting colonies and broth-culturing overnight, I isolated plasmid....

27 December 2020 3,596 5 View

MiRNA seq differential analysis?

Hello, I am writing to ask your help in regard to performing differential analysis to analyze miR RNA-seq data. I have miRNA seq data and I have analyzed in the following way- Initial QC - removed...

19 August 2020 5,105 7 View

How can I know the rank of each node in RPL in NetSim after simulation?

I am able to successfully run IOT RPL simulations in NetSim but in the results window, animator and plots I am not able to find the rank of the nodes. Please let me know how can I find this. Thanks

03 August 2020 3,630 3 View

How to calculate RECEIVER OPERATING CHARACTERISTICS(ROC) curves and area under the curve (AUC) of microRNA data?

Hello, I am having micro RNA data sets of control and patients now I am interested into analyzing micro RNA as a biomarker for a particular disease. I have already delta delta CT values for...

30 June 2020 7,858 2 View

How can i reduce primer dimers and get my cDNA PCR amplification done?

24 March 2020 1,525 3 View

I need to do Measurement of spectral emissivity by a FTIR spectrometer?

Please let me knw where i can complete this test in india

06 February 2019 6,227 2 View

How to apply statistical model in scenarios where dependent/response variable is not defined/labeled?

I have a scenario where many people are using a software and while utilizing they are assigned values between 0 to 1 based on their good or bad usage. e.g. good use would provide value to "usage"...

26 March 2018 300 4 View

What are the common steps involved in text analytics projects?

If i have to get the most possible generic steps of text analytics, what are the most commonly used steps for any text analysis model. Any help and your expert guidance/ suggestions are...

29 August 2017 9,968 8 View

Are you all really convinced for the adaption of Artificial Intelligence for future?

"The AI Takeover Is Coming" this is what is the news these days. Is it really a trend setter for future years. What is the impact over manual work due to this? just needed the audience thoughts...

29 August 2017 8,322 37 View

Dear all, I was wondering if anyone knows whether MGLtools and Autodock can run on Mac OS?

I have tried to download the 64-bit version of MGLtools however, as many times as I have downloaded and uninstall the programs, an error arises that the app needs to be updated. Thank you.

03 March 2021 5,997 2 View

I got 02 moderator values defining Johnson-Neyman significance regions. Is it an error?

Hi everyone, I am conducting research for my Master's thesis. I am using PROCESS by Johnson-Neyman to analyze my Moderator model. I test the relationship between Public Service Motivation and...

03 March 2021 2,350 2 View

Do I need to do linear regression before running mediation analysis using PROCESS MACRO by Hayes ?

I just wanted to check if I need to run a linear regression separately if I am using PROCESS MACRO to run mediation analysis. Thank you.

02 March 2021 4,359 3 View

APA 7th In-Text citations with two references with three authors with the same surname, but four different author?

I have an issue with APA 7th in-text citation. II have two papers where the first three authors are the same, and the fourth is different. I am using the Mendeley desktop app, and automatically it...

02 March 2021 790 3 View

Do I need to strip the PVDF membrane before staining it with colloidal gold?

Dear Colleagues, After running Western blot on PVDF membrane and detection using ECL, I would like to stain my PVDF with colloidal gold to be able to allign the ECL image with total proteins on...

02 March 2021 7,829 3 View

PACYC PCR not working after several attempts. Would anyone have any suggestions?

So, I have been trying to run a pACYC PCR which will be used later on for a Gibson Assembly. However the PCR is not working. I have already tried gradient PCR and changing extension time; however...

02 March 2021 1,146 2 View

What SPSS analysis should I run with one IV (with two levels) and two DVs?

Hi, I am trying to figure out which test would be most appropriate to run for the following question: Is there a difference in task blood pressure between smokers and non-smokers? There are two...

01 March 2021 8,235 2 View

OpenBCI eeg--has anyone used them?

I'm looking to try out an off-the-shelf EEG set for some exploratory work. Curious if anyone has used the OpenBCI, and how much set-up work is required. I've heard they are more difficult to get...

01 March 2021 4,128 3 View

How to address this issue in the analysis of Real-Time PCR results?

To dear Researchers, I was analyzing a series of concentration for estimation of Real-Time PCR efficiency. The concentration was 1:10. I used MS-excel to evaluate Slope. The result of slope was -8...

01 March 2021 8,683 4 View

Is there any expert in Smart PLS who can help me?

Dear colleagues, I have a manuscript that needs an expert in smart PLS to run the analysis, Is there anyone expert and ready to help me, please?

01 March 2021 8,212 3 View