I am working with haemophilus influenzae, as you know it need choclate agar to grow, but I must grow it in a medium without blood, any suggtion? can I

More Saeideh Momtaz's questions See All

How to solubilize GST tagged protein?

Hello, I was working to purify GST-tagged proteins expressed in BL21-DE3 strains. I was having some trouble with the formation of viscous stuff following sonication for the first couple of...

16 April 2023 7,584 3 View

What could be the reason for getting basal OCR too high (around 300-500 pmol/min) in Seahorse ATP rate assay?

I recently ran an ATP rate assay using the Seahorse XFp mini plate (8 wells). I got OCR values way higher than I typically see in publications. Basal OCR for control and experimental samples...

23 February 2023 8,256 3 View

Is there any technique to normalize Seahorse data without using BioTek Cytation 1 or 5 system?

Hello, We're going to perform a Seahorse assay using XFp 8-well system. The cell density will be 20,000 cells/well. We don't have the Cytation system to perform automated cell counts. As well as,...

19 February 2023 8,991 6 View

Current density in cathodic electrodeposition?

How can I determine appropriate current density value theoretically for cathodic electrodeposition process?

14 August 2022 6,395 1 View

Why the Sediment transport rate is different for total simulation time and the accumulated monthly sediment drift rate?

Dear All, I have an issue regarding a sediment transportation simulation. I have computed the sediment transport rate based on a 20 years wave climate, and simulated the sediment drift for the...

10 July 2022 8,926 2 View

Does anyone having problem with seeing only red puncta with PkhLC3 plasmid?

PKhLC3 plasmid is to observe the autophagic dynamics. The EGFP-Phluorin will disappear if the autophagosome fuses with the lysosome due to the acidic pH of the lysosome. So we expect to see only...

15 June 2022 4,605 0 View

RNA seq analysis using GSEA?

Hi everyone, I have a question and I was hoping to get some insight from you. I ran RNA-seq on my samples and I didn't have replicates, I used differentially expressed genes with a cut-off of...

01 June 2022 1,350 2 View

Does anyone having white precipitates after adding Pierce reagent?

Hello, I was using RIPA for protein lysis. I have recently seen a white precipitation formation after adding the Pierce reagent. We tried dilution of protein but still saw that white formation....

09 May 2022 9,170 6 View

TGF-β with or without carrier?

Hi everyone, I am planning to use TGF-β to transform my fibroblasts into CAFs. Most of the papers I have been reading have used TGF-β from R&D Systems, but none of them has mentioned whether they...

10 May 2021 733 3 View

Which CD44 variant can be used as intestinal stem cell marker for immunofluorescence?

We have IM7 that can recognize a common epitope for all the variants. It's no good for us for immunofluorescence. We will appreciate any advice or suggestions regarding this. Thank you!

03 May 2021 7,149 2 View

Do some Staphylococcus aureus strains have in vitro antimycobacterial activity ?

Some Staphylococcus aureus strains Inhibit the growth of Mycobacteria in Mueller Hinton Agar medium containing 10% OADC. Do some Staphylococcus aureus strains have in vitro antimycobacterial activity?

29 July 2024 10,023 2 View

Pink bacterial colonies?

I am cloning an overexpression plasmid with my protein of interest tagged with mScarlet. After transforming my ligated product into DH5α bacteria and plating on LB agar, I noticed colonies with a...

22 July 2024 5,953 6 View

Should all lactic acid bacteria be catalase negative? should all probiotic bacteria be catalase negative?

I am working on probiotic bacteria for fish, and I am trying to isolate probiotic bacteria like lactic acid bacteria or other probiotics from the gastrointestinal tract of fish using different...

12 July 2024 3,756 4 View

Can we change blood agar in the mCIM and eCIM protocols?

In the modified carbapenem inactivation method (mCIM) and EDTA-carbapenem inactivation method (eCIM) protocols, can we replace blood agar with another culture medium?

10 July 2024 6,352 0 View

Arabidopsis contamination in Hydroponic Culture ?

Hi I have a real problem growing Arabidopsis in hydroponic culture .I am using the bvoxes from Araponics and a Binder growth chamber with short day conditions and everything accurate .the seeds...

25 June 2024 9,151 6 View

Why I am not getting colony after transformation in Dh5alpha?

I am trying to do gibson assembly cloning. I have two fragments for gibson assembly cloning (plasmid backbone and insert). After gibson assembly reaction I have transformed this in E. coli....

19 June 2024 6,404 4 View

Pseudomonas aeruginosa colonies are too transparent: any tips on counting?

Hi everyone. I need to do a lot of colony counting tomorrow and when I was doing it today I’ve noticed that most of the colonies are too transparent - I have to shine bright light on them all the...

12 June 2024 711 3 View

Media Preparation protocol for Bifidobacteria Agar and Broth?

Hello everyone! I have been trying to develop a protocol for my thesis research which includes culturing and storing Bifidobacteria. I have come across many articles and studies related to the...

10 June 2024 4,152 1 View

I am working on antimicrobial effect of CBD (Cannabidiol), how should I prepare the stock which dissolve in Muller Hinton agar?

I am working on antimicrobial effect of CBD (Cannabidiol). As it is insoluble in water, I prepared a stock of 500 mg/ml (in DMSO). Now I have two problems: 1- I can not dissolve more than 500 mg...

10 June 2024 9,564 6 View

Which is the best media for revival of Pseudomonas aeruginosa?

Hi I am trying to revive Pseudomonas aeruginosa from frozen glycerol stock and also from cryobeads. But there was no bacterial growth. I used nutrient agar, tryptone soy agar to streak on. Also I...

09 June 2024 6,651 9 View