I would try CNPase, MBP (Myelin Basic Protein) or ABCA2(ATP-binding cassette sub-family A member 2 ) and Alexa dies for secondary antibodies

Hello

i agree with esearchers , also CD11b, CD40, B7 ,CD45, ICAM-1 and Anti-Microglia, clone 25F9 .

this link for some antibodies .

Anti-CD45 antibody [LT40] (Biotin)

http://www.abcam.com/CD45-antibody-LT40-Biotin-ab25155.html

Anti-CD45 antibody [LT40] (Biotin) (ab25155)

http://www.abcam.com/CD45-antibody-LT40-Biotin-ab25155.html

Anti-CD45 antibody [LT40] (PE/Cy5®) (ab25156)

http://www.abcam.com/CD45-antibody-LT40-PECy5-ab25156.html

Anti-Microglia, clone 25F9 from Merck Millipore

http://www.biocompare.com/9776-Antibodies/3034169-Anti-Microglia-clone-25F9/?ncatid=9776&ppim=3034169_1_1

Thanks Gankam, Fredrique and Saleh. This would be real help to me.

All the markers previously suggested are share with macrophages and other myeloid cells. If you are studying healthy tissues they should suffice, but under inflammatory conditions you should consider markers like P2ry12, 4d4 and Tmem119. Best !

Jorge: also the markers suggested are for either Flow cyto or IP.  I wanted to know microgllia cell marker suitable for immunoflorescence in brain tissue of chicken

There are antibodies suitable for both techniques. You should always consider testing Abs yourself to see if they work in your hands and particularly in your tissues.

Check this paper: http://www.ncbi.nlm.nih.gov/pubmed/24316888

Best

CX3CR1 has been used as a reporter gene in transgenic animals for microglia.  

Thanks, 

BU1-a

https://www.southernbiotech.com/MonoclonalDetails.aspx?catno=8365-09

From published work: We mapped the distribution of the three neuroglial cells, oligodendrocytes, astrocytes and microglia, in the chicken optic tectum using their specific markers, transferrin binding protein (TfBP), glial fibrillary acidic protein (GFAP), and Ricinus communis agglutinin-1 (RCA-1), respectively.

Article Glial cells in the chicken optic tectum