There are lots of primer sets available (e.g. actin, tubulin, calmodulin, etc.). Of course, they won't all work with every species/isolate. A good source, which gives primer sequences and references, is:

http://www.q-bank.eu/Fungi/DefaultInfo.aspx?Page=PrimerList

See also:

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4713107/

Hello Nasir,

I would suggest these primers:

SR6R: 5´ AAGWAAAAGTCGTAACAAGG 3´

5.8S: 5´CGCTGCGTTCTTCATCG 3´

These are ITS 1 Primers. The product you get is about 220-250bp long. Annealing Temp is 55°C. They work with all fungi. I´ve been using them for many years, also for sequence analysis.

Here are even more: http://sites.biology.duke.edu/fungi/mycolab/primers.htm

Best wishes

Dirk

Depends on what your purpose is!

Thank you for your inputs.. Cheers :)