animal is induced using stz (40 mg/kg) and nad(110mg/kg). when we checked the fasiting blood glucose one day it has decreased week later it has increased. pls clarify.
3 days after the induction of the dibetes using nicotinamide (110mg/kg) and streptozotocin(40mg/kg) the animal blood glucose was checked and it was treated with metformin(300 mg/kg). Glucose check was done for every 7 days with animal being fasted for 6 hours. first week FBG was found to be 168, 100 and 274. Metformin treatment was continued. after one week when animal checked with fasting blood glucose again it showed 600+.
Dear Gayathri,
In my view,you started your investigation sooner than diabetes establishment in the animals. Three days after STZ injection is suitable just for distinguishing diabetic from nondiabetic animals, but to confirm and establish diabetes in animal i recommend keeping diabetic animals at least for 2 weeks before starting investigation.
Regards,
Mehran
Dear Gayathri,
Please describe more details such as FBS levels, time of fasting, duration between diabetes induction and glucose concentration check. Each of mentioned parameter may cause of the fluctuation.
More information would be require to give possible answers to your observations.
metformin is a drug that is used in the treatment but it is not the only one as its mechanism related to hepatic glucose release and reduction in metabolic resistance
more information would be required including type of diabetes , the animals variation may have a role in the fluctuation
3 days after the induction of the dibetes using nicotinamide (110mg/kg) and streptozotocin(40mg/kg) the animal blood glucose was checked and it was treated with metformin(300 mg/kg). Glucose check was done for every 7 days with animal being fasted for 6 hours. first week FBG was found to be 168, 100 and 274. Metformin treatment was continued. after one week when animal checked with fasting blood glucose again it showed 600+.
Dear Gayathri,
In my view,you started your investigation sooner than diabetes establishment in the animals. Three days after STZ injection is suitable just for distinguishing diabetic from nondiabetic animals, but to confirm and establish diabetes in animal i recommend keeping diabetic animals at least for 2 weeks before starting investigation.
Regards,
Mehran
Hi Gayathri, I was curious to know if you used similar method for estimation of fasting blood glucose (FBG) before and after treatment and also if the blood was drawn from similar area everytime (like tail vein). I work on SD rats and while checking FBG with the help of glucometer, I once got a high value for one of the rats but when I repeated, I got a value in the normal range. Also, from the same rat, I have seen FBG value to be higher when blood is collected from retro-orbital plexus than that obtained from tail vein.
Is the number of animal showing FBG>600 statistically significant ie how many of the animals displayed high FBG? (Its strange if all the rats are showing this great value!).
Did you observe any noticeable difference in eating/ drinking behaviour or any stress which would have might resulted in fluctuations?
all above point should be considered but it may be to complete destruction of pancreas
How and when is Metformin administered in your study?
An frequently neglected issue in drug studies are the pharmacodynamics of the drug and the relationship between its metabolism and meals.
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