The Circular dichroism shows negative ellipticity but on visualizing in SEM and TEM it is not possible to see the twist of fibrils during the self-assembly
Dinesh Kumar Duraisamy
The antipodes of twisted fibrils cannot be determined using TEM and SEM. You will not distinguish between the left and right hands with your eyes if their morphology from above and below is the same. Also, you can't tell which antipode you see if you don't see another side by side. Alpha and beta glucose will look like white crystals under the microscope.
Negative ellipticity in Circular Dichroism (CD) indicates chiral molecular structures with left-handed twists. Morphology visualization might not capture twists in fibrils due to limited resolution. Twists could be finer than imaging capabilities or occur at scales not observable. Higher-resolution techniques like electron microscopy could reveal fibril twists underlying negative ellipticity.
Asma Iqbal
In TEM you will see fibril bends, but how can you prove that they are with negative ellipticity?
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