I am using MDA MB 468 cells. I want to isolated the plasma membrane as I want to study a protein that is membranous. Can you suggest best ways?

Swapnil Shinde @Swapnil_Shinde3

06 June 2015 1 5K Report

Also, What can be the best control for western blotting?

Ravi Pottathil

Please see this relatively simple protocol
https://www.lifetechnologies.com/us/en/home/life-science/protein-biology/protein-biology-learning-center/protein-biology-resource-library/protein-biology-application-notes/efficient-mammalian-membrane-protein-extraction.html

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