I am testing a great number of recently arrived refugees/ immigrants for polio seroprotection: what is the best method and what the levels of seroprotection?

Ioanna OR Joanna D. Pavlopoulou @Ioanna-Or-Joanna-Pavlopoulou

23 October 2012 1 2K Report

Immunity

ELISA is the best method for screen. The level of seroprotection is individual, dependent of each infection (virus, bacterium).

Badges
Science topic

More Ioanna OR Joanna D. Pavlopoulou's questions See All

How to eliminate the carry-over effect in GC-MS/MS system?

Hi, I am a PhD student working at Jagiellonian University, Faculty of Chemistry. My work is related to the development of new analytical methods for the quantification of short-chain fatty acids...

22 July 2024 5,492 6 View

How to prepare bacterial conditioned media to study the effect of bacterial secretome?

I would like to investigate the effect of bacterial secretome on cancer cells. How do you prepare conditioned media from bacteria?? conditions- time points - media I am looking for the best way...

17 July 2024 3,785 9 View

Self discharge of freshly made NMC half cells and voltage stabilizes at 3.3-3.5 V vs Li/Li+ during charging. What can affect it?

I am making Li half cells using NMC as cathode. The OCV is around 3 V but it self discharges and during charging the voltage increases to 3.5 V and then drops to 3.3-3.4 V and stays there. I have...

23 June 2024 842 3 View

How should I dilute substance A? How can I calculate the dose to give to rats? Can you help with this issue?

Twenty one rats received 1.2 mg/kg A substance (MW:294.98 g/mol) dissolved in 1% Tween 80 by oral gavage for 14 consecutive days. How should I dilute substance A? How can I calculate the dose to...

11 March 2024 6,377 0 View

Volcanic Ash in modern concrete and cementitious materials?

I am writing a paper summarizing my research results on the possibility of usage of volcanic ash in modern cementites materials on the example of the tests of composits with particular (Japanese)...

21 February 2024 5,797 0 View

How to achieve RNA extraction of nucleus and cytoplasm in SH-SY5Y cells?

I have tried to achieve a separation of the nucleus and cytoplasm by using RLN buffer+ NP40 (0.2%), and then proceeding with GeneMATRIX universal RNA purification kit (the same used for total RNA...

04 February 2024 7,172 2 View

How to calculate DBH for trees with multiple dead and live stems in the same tree?

I am trying to calculate DBH (diameter at breast height) in order to determine the demography and basal area of trees. However, some of the trees have multiple stems which makes it difficult to...

23 January 2024 845 0 View

ReserchGate misatributes some citations to me. What should I do?

The site shows that my works are cited in some papers, however when I checked those papers, I found no sign of being referenced (on the other hand my work is referenced in completely different...

22 January 2024 3,766 2 View

Are you interested to collaborate within the new research project "Education in the AI era"?

I'm looking for hard-working researchers for the new research project "EDUCATION IN THE AI ERA." I would like to create the survey on Google and do international research. If you are interested in...

06 January 2024 5,488 21 View

Why I'm losing plasmid after digest with restriction enzymes?

Hi everyone, I'm currently working with a plasmid called pBI101, I isolated this plasmid from a plate then I did the extraction with mini prep protocol and cut it with SmaI at 25°C incubation...

17 November 2023 6,980 3 View

What precautions should be taken while handling S. aureus enterotoxin Type B in the lab?

I would like to understand potential safety concerns while handling SEB in the lab. Especially while working in animal house facility. Would like to know precautions for handling. Sigma MSDS...

07 August 2024 6,034 3 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

What is the best sampling strategy?

I am conducting a qualitative study that uses interviews to investigate the perceptions of teachers about a particular leadership practice and I am focusing on 3 schools which have a total number...

01 August 2024 8,457 10 View

Is it possible to do transient simulation in HFSS in presence of magnetic biasing?

I have designed magnetic material with different biasing conditions in HFSS. Now I want to give an RF AC signal and do a transient simulation in HFSS. Is it possible to do in HFSS? Please help me...

24 July 2024 9,319 5 View

What are the strategies to Enhance IgG-Producing Plasma Cells in mice for Monoclonal Antibody Development?

I have immunized BalB/C mice with a protein using the intradermal (ID) method with Complete Freund's Adjuvant (CFA) and Incomplete Freund's Adjuvant (IFA), following a 14-day interval and three...

22 July 2024 9,160 2 View

ANCOVA for between-subject design?

Hi, I will run an experiment where reaction time will be the dependent variable, tested in 2 conditions (with the same participants). I want to control for a confounding variable (individual...

20 July 2024 1,943 1 View

I am working on III-V based tandem solar cells.Can anyone explain that solar cells work under forward or reverse biased conditions?

I want to know that n-doped side of solar cell is connected with positive or negative electrode.

20 July 2024 1,348 2 View

Charge transfer characteristics of inverted perovskite leds?

When testing devices based on ITO/ZnO/CsPbBr3/TFB/MoO3/Ag structures, I found that the current density of the device always increases sharply to 1000mA/cm2 at about 3V bias, and the device does...

16 July 2024 4,291 0 View

How can I extract the mathematical equation from existing Neural Network Model?

There exists a neural network model designed to predict a specific output, detailed in a published article. The model comprises 14 inputs, each normalized with minimum and maximum parameters...

14 July 2024 2,714 3 View

Can ROBINS-I be used to measure risk of bias in single-arm studies?

I am doing a systematic review, and I am measuring risk of bias with RoB2 for RCT, and ROBINS-I for non RCT. My questions is, for single arm studies, can I use ROBINS-I? I am not sure how to...

14 July 2024 3,570 5 View