biopolymer/gene delivery
Dear Ihsan,
Why not doing a simple filtration before freeze-drying. Regards
hmmmm agreed with you...but I was confused about the chemical composition of precipitated polymer and the soluble species.
did you made any experimental characterization to prove the nature of the precipitate and the soluble compounds ? Regards
Dear Abdelkader
not yet....I am proceeding to investigate the chemical nature of precipitate and soluble part. thanks for your interest.
I want to carried out Michal type addition reaction, but zwitterion formation inhibit nucleophilicity of amine group. So how I can ceased zwitterion formation.. thanks for your attention.
09 October 2016 8,474 5 View
Hello everyone. we are working on synthetic polymer for gene delivery, where buffering capacity is important factors for gene transfection. buffering capacity is given by following...
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06 August 2024 1,350 4 View
is there any other way to heat a polymer evenly other than oil bath if so what are those and how to use it
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I aim to be as skeptical as possible regarding whether a pair of orthologous genes results in the same phenotype in their different but related bacterial organisms under similar environmental...
05 August 2024 6,787 4 View
I am performing ligation of the plasmid and a target gene. The steps I have taken are: 1. Double digestion of the plasmid and target gene 2. Ligation of the plasmid with the target gene 3....
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kindly reply me. Thanking you in advance.
05 August 2024 7,727 4 View
Is it the "elution buffer" or the "dialysis buffer"? Note: I'll be using NanoDrop OneC
01 August 2024 967 3 View
I have an RNA-seq data that I have analysed using Limma-voom and have extracted the gene IDs, log2FC and the p-values. At p value < 0.05, I have over 10,000 DEGs, however, when I run the GO...
31 July 2024 225 2 View
I am currently working on a project involving liposomes and need to determine the maximum volume of siRNA that can be added to a 2.5 mL liposome solution with a total lipid concentration of 10...
30 July 2024 6,420 1 View